The enzyme concentration = (4 mg/l)/(40,000 g/mol) = 0.10mM
(10-7 M).
The last two columns were calculated using equations from the Enzyme
Kinetics page.
Graph of the calculated results:
The Km = 2.0 mM; Vmax = 0.5 mM/min.
The kcat = Vmax/[enzyme] = (0.5 mM/min)/(0.10mM)
= 5¥103 min-1.
MM.xls is the Excel spreadsheet used to calculate and graph
these results; it can be copied to your disk.