Lesson 2:
Introduction to Theory, Practice of Radioligand Binding

Radioactivity, Radioligands and Binding Assays

How to Separate Bound from Free

   Equilibrium dialysis

   Centrifugation

   Filtration

Total vs. free concentrations of ligand. Ligand depletion

Radioactivity

   Specific Radioactivity

   Radioactive decay

Selecting the radioligand

   What chemical structure: agonist vs. antagonist

   Choice of isotope

The Poisson distribution

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