BLASTP2 Result
of 
Bork Group's Advanced BLAST2 Search Service at 
EMBL.
back to BLAST2
BLASTP 2.0MP-WashU [16-Dec-1999] [irix6-r10k-L64 23:35:48 16-Dec-1999]
Copyright (C) 1996-1999 Washington University, Saint Louis, Missouri USA.
All Rights Reserved.
Reference: Gish, W. (1996-1999) http://blast.wustl.edu
Query= FTSA_ECOLI
(420 letters)
Echofilter:
>Filtered+0
MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSRGMDKGGVNDLESVVKC
VQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEEEVTQEDVENVVHTAKSVR
VRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHLITCHNDMAKNIVKAVERCGL
KVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDIAVYTGGALRHTKVIPYAGNVVT
SDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVEVPSVGGRPPRSLQRQTLAEVIEPR
YTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVLTGGAAQIEGLAACAQRVFHTQVRIGA
PLNITGLTDYAQEPYYSTAVGLLHYGKESHLNGEAEVEKRVTASVGSWIKRLNSWLRKEF
Database: pdb Release 27.Feb.2002
20,403 sequences; 4,491,068 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done
Query sequence and locally-aligned HSPs from database sequences
0 90 180 270 360
| | | | | |420
FTSA_ECOLI | __________________________________________________
^ v pdb|1E4F|1E4F-T | ______________________________________________
^ v pdb|1E4G|1E4G-T | ______________________________________________
^ v pdb|1JCE|1JCE-A | ____________________
^ v pdb|1JCF|1JCF-A | ____________________
^ v pdb|1JCG|1JCG-A | ____________________
^ v pdb|1AO0|1AO0-A | _________________________
^ v pdb|1GPH|1GPH-1 | _________________________
^ v pdb|1HY5|1HY5-A | ___________
^ v pdb|1CEE|1CEE-B | _____
Alignments:
^ = pdb|1E4F|1E4F-T FTSA (APO FORM) FROM THERMOTOGA MARITIMA
Length = 419
0 90 180 270 360
| | | | | |419
pdb|1E4F|1E4F-T | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 233 (87.1 bits), Expect = 2.7e-18, P = 2.7e-18
Identities = 82/392 (20%), Positives = 178/392 (45%)
Query: 1 MIKATDRKLVVGLEIGTAKVAALV-GEVLPDGMVNIIGVGSCPSRGMDKGGVNDLESVVK 59
MI + ++IG+ + LV G+ D + S SRG+D+G + D + +
Sbjct: 1 MIDLSKTVFYTSIDIGSRYIKGLVLGK--RDQEWEALAFSSVKSRGLDEGEIKDAIAFKE 58
Query: 60 CVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVP--ISEEE--VTQEDVENVVHT 115
V + + E + S ++ +S +S + E ++ EE+ +T D+ + + +
Sbjct: 59 SVNTLLKELEEQLQKSLRSDFV-ISFSSVSFEREDTVIERDFGEEKRSITL-DILSEMQS 116
Query: 116 AKSVRVRDEHRV-LHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHLITCHNDMAKNIVKA 174
++++ + LH+ + Y +D + + NP+ + ++ + I + +
Sbjct: 117 EALEKLKENGKTPLHIFSKRYLLDDERIVFNPLDMKASKIAIEYTSIVVPLKVYEMFYNF 176
Query: 175 VERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDIAVYTGGALRHTKVIPY 234
++ QL + ++++ VLT E++ GV VV++G + Y G +P
Sbjct: 177 LQDTVKSPFQLKSSLVSTAEGVLTTPEKDRGVVVVNLGYNFTGLIAYKNGVPIKISYVPV 236
Query: 235 AGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVEVPSVGGRPPRSLQRQTLA 294
V D++ T ++E + + HG A+ + + K+E ++ + G ++ + L+
Sbjct: 237 GMKHVIKDVSAVLDTSFEESERLIITHGNAVYNDL-KEEEIQYRGLDGNTIKTTTAKKLS 295
Query: 295 EVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVLTGGAAQIEGLAACAQRVFHT 354
+I R E+++ + +++ K+ ++G + + G+VLTGG A+I + A VF +
Sbjct: 296 VIIHARLREIMSKSKKFFREVEAKIVEEG-EIGIPGGVVLTGGGAKIPRINELATEVFKS 354
Query: 355 QVRIGAPLN-----ITGLTDYAQEPYYSTAVG 381
VR G N I + A +P ++ A G
Sbjct: 355 PVRTGCYANSDRPSIINADEVANDPSFAAAFG 386
^ = pdb|1E4G|1E4G-T FTSA (ATP-BOUND FORM) FROM THERMOTOGA MARITIMA
Length = 419
0 90 180 270 360
| | | | | |419
pdb|1E4G|1E4G-T | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 233 (87.1 bits), Expect = 2.7e-18, P = 2.7e-18
Identities = 82/392 (20%), Positives = 178/392 (45%)
Query: 1 MIKATDRKLVVGLEIGTAKVAALV-GEVLPDGMVNIIGVGSCPSRGMDKGGVNDLESVVK 59
MI + ++IG+ + LV G+ D + S SRG+D+G + D + +
Sbjct: 1 MIDLSKTVFYTSIDIGSRYIKGLVLGK--RDQEWEALAFSSVKSRGLDEGEIKDAIAFKE 58
Query: 60 CVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVP--ISEEE--VTQEDVENVVHT 115
V + + E + S ++ +S +S + E ++ EE+ +T D+ + + +
Sbjct: 59 SVNTLLKELEEQLQKSLRSDFV-ISFSSVSFEREDTVIERDFGEEKRSITL-DILSEMQS 116
Query: 116 AKSVRVRDEHRV-LHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHLITCHNDMAKNIVKA 174
++++ + LH+ + Y +D + + NP+ + ++ + I + +
Sbjct: 117 EALEKLKENGKTPLHIFSKRYLLDDERIVFNPLDMKASKIAIEYTSIVVPLKVYEMFYNF 176
Query: 175 VERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDIAVYTGGALRHTKVIPY 234
++ QL + ++++ VLT E++ GV VV++G + Y G +P
Sbjct: 177 LQDTVKSPFQLKSSLVSTAEGVLTTPEKDRGVVVVNLGYNFTGLIAYKNGVPIKISYVPV 236
Query: 235 AGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVEVPSVGGRPPRSLQRQTLA 294
V D++ T ++E + + HG A+ + + K+E ++ + G ++ + L+
Sbjct: 237 GMKHVIKDVSAVLDTSFEESERLIITHGNAVYNDL-KEEEIQYRGLDGNTIKTTTAKKLS 295
Query: 295 EVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVLTGGAAQIEGLAACAQRVFHT 354
+I R E+++ + +++ K+ ++G + + G+VLTGG A+I + A VF +
Sbjct: 296 VIIHARLREIMSKSKKFFREVEAKIVEEG-EIGIPGGVVLTGGGAKIPRINELATEVFKS 354
Query: 355 QVRIGAPLN-----ITGLTDYAQEPYYSTAVG 381
VR G N I + A +P ++ A G
Sbjct: 355 PVRTGCYANSDRPSIINADEVANDPSFAAAFG 386
^ = pdb|1JCE|1JCE-A MREB FROM THERMOTOGA MARITIMA
Length = 344
0 70 140 210 280
| | | | | |344
pdb|1JCE|1JCE-A | __________________________________________________
Local hits (HSPs) | ________________________
Score = 107 (42.7 bits), Expect = 0.00030, P = 0.00030
Identities = 49/178 (27%), Positives = 81/178 (45%)
Query: 182 VDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDIAVYTGGALRHTKVIPYAGNVVTS 241
+++ + A + S+ +V E G VVDIGGGT ++AV + G++ + I AG+ +
Sbjct: 129 IEEPMAAAIGSNLNV----EEPSGNMVVDIGGGTTEVAVISLGSIVTWESIRIAGDEMDE 184
Query: 242 DIA------YAFGTPPSDAEAIKVRHGCALGSIVGKDESVEVPSVGGRPPRSLQRQ-TL- 293
I Y AE +K+ G S +++ +E G L R+ TL
Sbjct: 185 AIVQYVRETYRVAIGERTAERVKIEIGNVFPS--KENDELETTVSGIDLSTGLPRKLTLK 242
Query: 294 -AEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVLTGGAAQIEGLAACAQR 350
EV E + ++ +V E + EK + V + GI LTGG + + GL Q+
Sbjct: 243 GGEVREALRSVVVAIV-ESVRTTLEKTPPELVSDIIERGIFLTGGGSLLRGLDTLLQK 299
^ = pdb|1JCF|1JCF-A MREB FROM THERMOTOGA MARITIMA, TRIGONAL
Length = 344
0 70 140 210 280
| | | | | |344
pdb|1JCF|1JCF-A | __________________________________________________
Local hits (HSPs) | ________________________
Score = 107 (42.7 bits), Expect = 0.00030, P = 0.00030
Identities = 49/178 (27%), Positives = 81/178 (45%)
Query: 182 VDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDIAVYTGGALRHTKVIPYAGNVVTS 241
+++ + A + S+ +V E G VVDIGGGT ++AV + G++ + I AG+ +
Sbjct: 129 IEEPMAAAIGSNLNV----EEPSGNMVVDIGGGTTEVAVISLGSIVTWESIRIAGDEMDE 184
Query: 242 DIA------YAFGTPPSDAEAIKVRHGCALGSIVGKDESVEVPSVGGRPPRSLQRQ-TL- 293
I Y AE +K+ G S +++ +E G L R+ TL
Sbjct: 185 AIVQYVRETYRVAIGERTAERVKIEIGNVFPS--KENDELETTVSGIDLSTGLPRKLTLK 242
Query: 294 -AEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVLTGGAAQIEGLAACAQR 350
EV E + ++ +V E + EK + V + GI LTGG + + GL Q+
Sbjct: 243 GGEVREALRSVVVAIV-ESVRTTLEKTPPELVSDIIERGIFLTGGGSLLRGLDTLLQK 299
^ = pdb|1JCG|1JCG-A MREB FROM THERMOTOGA MARITIMA, AMPPNP
Length = 344
0 70 140 210 280
| | | | | |344
pdb|1JCG|1JCG-A | __________________________________________________
Local hits (HSPs) | ________________________
Score = 107 (42.7 bits), Expect = 0.00030, P = 0.00030
Identities = 49/178 (27%), Positives = 81/178 (45%)
Query: 182 VDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDIAVYTGGALRHTKVIPYAGNVVTS 241
+++ + A + S+ +V E G VVDIGGGT ++AV + G++ + I AG+ +
Sbjct: 129 IEEPMAAAIGSNLNV----EEPSGNMVVDIGGGTTEVAVISLGSIVTWESIRIAGDEMDE 184
Query: 242 DIA------YAFGTPPSDAEAIKVRHGCALGSIVGKDESVEVPSVGGRPPRSLQRQ-TL- 293
I Y AE +K+ G S +++ +E G L R+ TL
Sbjct: 185 AIVQYVRETYRVAIGERTAERVKIEIGNVFPS--KENDELETTVSGIDLSTGLPRKLTLK 242
Query: 294 -AEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVLTGGAAQIEGLAACAQR 350
EV E + ++ +V E + EK + V + GI LTGG + + GL Q+
Sbjct: 243 GGEVREALRSVVVAIV-ESVRTTLEKTPPELVSDIIERGIFLTGGGSLLRGLDTLLQK 299
^ = pdb|1AO0|1AO0-A GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE
FROM B. SUBTILIS COMPLEXED WITH ADP AND GMP
Length = 459
0 100 200 300 400
| | | | | |459
pdb|1AO0|1AO0-A | __________________________________________________
Local hits (HSPs) | ______________________
Score = 81 (33.6 bits), Expect = 0.35, P = 0.29
Identities = 59/227 (25%), Positives = 93/227 (40%)
Query: 123 DEHRVLHVIPQEYAIDYQEGIKNPVG-LSGVRMQAKVHLITCHNDMAKNIVKAVERCGLK 181
D + H+I + ++ IKN + L G A LI +M + A++ GL+
Sbjct: 127 DTEVLAHLIKRSGHFTLKDQIKNSLSMLKG----AYAFLIMTETEM----IVALDPNGLR 178
Query: 182 VDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDIAVYTGGAL----RHTKVIPYAGN 237
L + +Y V +E C D+ G T V G L K ++ N
Sbjct: 179 --PLSIGMMGDAYVVASE------TCAFDVVGATYLREVEPGEMLIINDEGMKSERFSMN 230
Query: 238 VVTS--DIAYA-FGTPPSDAEAIKVRHGCA--LGSIVGKDESVEVPSVGGRPPRSLQRQT 292
+ S + Y F P S+ + I V H LG ++ ++ +VE V G P S+
Sbjct: 231 INRSICSMEYIYFSRPDSNIDGINV-HSARKNLGKMLAQESAVEADVVTGVPDSSISAAI 289
Query: 293 -LAEVIE-PRYTELLN--LVNEEILQLQEKLRQQGVKHHLAA--GIV 333
AE P L+ V +Q + LR+QGV+ L+A G+V
Sbjct: 290 GYAEATGIPYELGLIKNRYVGRTFIQPSQALREQGVRMKLSAVRGVV 336
^ = pdb|1GPH|1GPH-1 GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE
(E.C.2.4.2.14)
Length = 465
0 100 200 300 400
| | | | | |465
pdb|1GPH|1GPH-1 | __________________________________________________
Local hits (HSPs) | ______________________
Score = 81 (33.6 bits), Expect = 0.35, P = 0.30
Identities = 59/227 (25%), Positives = 93/227 (40%)
Query: 123 DEHRVLHVIPQEYAIDYQEGIKNPVG-LSGVRMQAKVHLITCHNDMAKNIVKAVERCGLK 181
D + H+I + ++ IKN + L G A LI +M + A++ GL+
Sbjct: 127 DTEVLAHLIKRSGHFTLKDQIKNSLSMLKG----AYAFLIMTETEM----IVALDPNGLR 178
Query: 182 VDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDIAVYTGGAL----RHTKVIPYAGN 237
L + +Y V +E C D+ G T V G L K ++ N
Sbjct: 179 --PLSIGMMGDAYVVASE------TCAFDVVGATYLREVEPGEMLIINDEGMKSERFSMN 230
Query: 238 VVTS--DIAYA-FGTPPSDAEAIKVRHGCA--LGSIVGKDESVEVPSVGGRPPRSLQRQT 292
+ S + Y F P S+ + I V H LG ++ ++ +VE V G P S+
Sbjct: 231 INRSICSMEYIYFSRPDSNIDGINV-HSARKNLGKMLAQESAVEADVVTGVPDSSISAAI 289
Query: 293 -LAEVIE-PRYTELLN--LVNEEILQLQEKLRQQGVKHHLAA--GIV 333
AE P L+ V +Q + LR+QGV+ L+A G+V
Sbjct: 290 GYAEATGIPYELGLIKNRYVGRTFIQPSQALREQGVRMKLSAVRGVV 336
^ = pdb|1HY5|1HY5-A CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF YOPE-YERSINIA
PESTIS GAP EFFECTOR PROTEIN.
Length = 136
0 30 60 90 120
| | | | | |136
pdb|1HY5|1HY5-A | __________________________________________________
Local hits (HSPs) | ___________________________________
Score = 66 (28.3 bits), Expect = 1.9, P = 0.85
Identities = 29/101 (28%), Positives = 41/101 (40%)
Query: 290 RQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVLTGGAAQIEG--LAAC 347
+Q AE + P+Y + LN ++ E+LQ G L I G Q G L A
Sbjct: 18 KQLAAETL-PKYMQQLNSLDAEMLQKNHDQFATG-SGPLRGSITQCQGLMQFCGGELQAE 75
Query: 348 AQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKE 388
A + +T V G P + G A Y ++ V L E
Sbjct: 76 ASAILNTPV-CGIPFSQWGTIGGAASAYVASGVDLTQAANE 115
^ = pdb|1CEE|1CEE-B SOLUTION STRUCTURE OF CDC42 IN COMPLEX WITH THE GTPASE
BINDING DOMAIN OF WASP
Length = 59
0 20 40
| | | |59
pdb|1CEE|1CEE-B | __________________________________________________
Local hits (HSPs) | ______________________________________
Score = 46 (21.3 bits), Expect = 5.8, P = 1.0
Identities = 15/50 (30%), Positives = 24/50 (48%)
Query: 29 PDGMVNIIGVGSCPSRGMDKGGVN-DLESVVKCVQRAIDQAELMADCQIS 77
P G ++ VG P G D ++ DL S+ + I +A+L D + S
Sbjct: 12 PSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFS--RAGISEAQL-TDAETS 58
Parameters:
matrix=BLOSUM62
E=10
V=50
B=50
hspmax=10
filter=seg
echofilter
stats
qtype
qres
ctxfactor=1.00
Query ----- As Used ----- ----- Computed ----
Frame MatID Matrix name Lambda K H Lambda K H
+0 0 BLOSUM62 0.317 0.135 0.386 same same same
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
Query
Frame MatID Length Eff.Length E S W T X E2 S2
+0 0 420 420 10. 66 3 11 22 0.21 34
34 0.22 37
Statistics:
Query Expected Observed HSPs HSPs
Frame MatID High Score High Score Reportable Reported
+0 0 60 (27.5 bits) 83 (38.0 bits) 9 9
Query Neighborhd Word Excluded Failed Successful Overlaps
Frame MatID Words Hits Hits Extensions Extensions Excluded
+0 0 8686 3779186 775719 2998339 5128 2
Database: /dove2/data/db/pdb
Title: pdb Release 27.Feb.2002
Release date: 27.Feb.2002
Posted date: 11:04:19 PM MET Feb 27, 2002
Last modified: unknown
First created: unknown
Format: BLAST-1.4
# of letters in database: 4,491,068
# of sequences in database: 20,403
# of database sequences satisfying E: 9
No. of states in DFA: 563 (110 KB)
Total size of DFA: 291 KB (320 KB)
Time to generate neighborhood: 0.01u 0.00s 0.01t Elapsed: 00:00:00
No. of threads or processors used: 4
Search cpu time: 6.39u 0.24s 6.63t Elapsed: 00:00:02
Total cpu time: 6.45u 0.30s 6.75t Elapsed: 00:00:03
Start: Tue Apr 16 17:25:31 2002 End: Tue Apr 16 17:25:34 2002
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