Links: BOTTOM PredictProtein Burkhard Rost




Results from PredictProtein for predict_h25944

TOC for file /home/phd/server/work/predict_h25944

  1. The following information has been received by the server (TOC)
  2. PROSITE motif search (A Bairoch; P Bucher and K Hofmann) (TOC)
  3. ProDom domain search (E Sonnhammer; Corpet, Gouzy, D Kahn) (TOC)
  4. PHD information about accuracy (TOC)
  5. PHD predictions (TOC)

END of TOC




BEG of results for file /home/phd/server/work/predict_h25944


The following information has been received by the server


reference predict_h25944 (Jan 23, 2001 05:04:04)
reference pred_h25944 (Jan 23, 2001 05:04:13)
PPhdr from: pagomez@cnb.uam.es
PPhdr resp: MAIL
PPhdr orig: HTML
PPhdr want: HTML
PPhdr password(###)
prediction of: - secondary structure   (PHDsec)-
return no alignment
ret html
ret html perline=60
# default: single protein sequence description=curso1
RLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCS
QSWGRSSKQWEDPSQASKVCQRLNCGVPLSLGPFLVT
YTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLE


PROSITE motif search (A Bairoch; P Bucher and K Hofmann)


TOP - BOTTOM - ProSite
-------------------------------------------------------------
Pattern-ID: ASN_GLYCOSYLATION PS00001 PDOC00001
Pattern-DE: N-glycosylation site
Pattern:    N[^P][ST][^P]
   92       NCSH

Pattern-ID: PKC_PHOSPHO_SITE PS00005 PDOC00005
Pattern-DE: Protein kinase C phosphorylation site
Pattern:    [ST].[RK]
   43       SSK

Pattern-ID: CK2_PHOSPHO_SITE PS00006 PDOC00006
Pattern-DE: Casein kinase II phosphorylation site
Pattern:    [ST].{2}[DE]
   3        SWYD
   96       SRND
   107      TCLE

Pattern-ID: MYRISTYL PS00008 PDOC00008
Pattern-DE: N-myristoylation site
Pattern:    G[^EDRKHPFYW].{2}[STAGCN][^P]
   63       GVPLSL
   85       GQLGSF



ProDom domain search (E Sonnhammer; Corpet, Gouzy, D Kahn)


TOP - BOTTOM - ProDom - MView
Identities computed with respect to: (query) prot
Colored by: consensus/70% and property
HSP processing: ranked
                   1 [        .         .         .         .         :         . 60 
  prot               RLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQWEDPSQASKVCQRL    
1 PD012755           QLSWEDPGLQMRLSGSGCRCQGQLEVQMSSRWHPVCSQSWGGVSPYWKNPRQAGKVCQQL    
2 PD002499           ------PKGQIRLVGGSSPCEGRVEVHMRGQWGTVCDSNWTRSEAQWE--AQWEVVCREL    
  consensus/100%     ......PthQhRLstuss.CpGplEV.hpstWt.VCspsWstsp..Wc..tQhthVCppL    
  consensus/90%      ......PthQhRLstuss.CpGplEV.hpstWt.VCspsWstsp..Wc..tQhthVCppL    
  consensus/80%      ......PthQhRLstuss.CpGplEV.hpstWt.VCspsWstsp..Wc..tQhthVCppL    
  consensus/70%      ......PthQhRLstuss.CpGplEV.hpstWt.VCspsWstsp..Wc..tQhthVCppL    

                  61          .         .         .         1         ] 110
  prot               NCGVPLSLGPFLVTYTPQSSIICYGQXXSFSNCSHSRNDMCHSLGLTCLE    
1 PD012755           GCRPPLKLGSAPPDYEPQWAIICHGQ--SFSNCNHSRNDQCHSLGLICLE    
2 PD002499           GCGDAVSV------------------------------------------    
  consensus/100%     sCtsslpl..................  ......................    
  consensus/90%      sCtsslpl..................  ......................    
  consensus/80%      sCtsslpl..................  ......................    
  consensus/70%      sCtsslpl..................  ......................    
--- ------------------------------------------------------------
--- 
--- Again: these results were obtained based on the domain data-
--- base collected by Daniel Kahn and his coworkers in Toulouse.
--- 
--- PLEASE quote: 
---       F Corpet, J Gouzy, D Kahn (1998).  The ProDom database
---       of protein domain families. Nucleic Ac Res 26:323-326.
--- 
--- The general WWW page is on:
----      ---------------------------------------
---       http://www.toulouse.inra.fr/prodom.html
----      ---------------------------------------
--- 
--- For WWW graphic interfaces to PRODOM, in particular for your
--- protein family, follow the following links (each line is ONE
--- single link for your protein!!):
--- 
http://www.toulouse.inra.fr/prodom/cgi-bin/ReqProdomII.pl?id_dom1=PD012755 ==> multiple alignment, consensus, PDB and PROSITE links of domain PD012755
http://www.toulouse.inra.fr/prodom/cgi-bin/ReqProdomII.pl?id_dom2=PD012755 ==> graphical output of all proteins having domain PD012755
http://www.toulouse.inra.fr/prodom/cgi-bin/ReqProdomII.pl?id_dom1=PD002499 ==> multiple alignment, consensus, PDB and PROSITE links of domain PD002499
http://www.toulouse.inra.fr/prodom/cgi-bin/ReqProdomII.pl?id_dom2=PD002499 ==> graphical output of all proteins having domain PD002499
--- 
--- NOTE: if you want to use the link, make sure the entire line
---       is pasted as URL into your browser!
--- 
--- END of PRODOM
--- ------------------------------------------------------------


PHD information about accuracy


****************************************************************************
*                                                                          *
*    PHD: Profile fed neural network systems from HeiDelberg               *
*    ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~               *
*                                                                          *
*    Prediction of:			                                   *
* 	secondary structure,   			   by PHDsec		   *
* 	solvent accessibility, 			   by PHDacc		   *
* 	and helical transmembrane regions, 	   by PHDhtm		   *
*                                                                          *
*    Author:             						   *
*	Burkhard Rost							   *
*       EMBL, 69012 Heidelberg, Germany					   *
*       Internet: Rost@EMBL-Heidelberg.DE				   *
*                                                                          *
*    All rights reserved.                                                  *
*                                                                          *
****************************************************************************
*                                                                          *
*    The network systems are described in:   	                     	   *
*                                                                          *
*    PHDsec:    B Rost & C Sander: JMB, 1993, 232, 584-599.		   *
*    		B Rost & C Sander: Proteins, 1994, 19, 55-72.		   *
*    PHDacc:  	B Rost & C Sander: Proteins, 1994, 20, 216-226.		   *
*    PHDhtm:  	B Rost et al.: 	   Prot. Science, 1995, 4, 521-533.	   *
*                                                                          *
****************************************************************************


PHD predictions


TOP - BOTTOM - PHD

PHD predictions for predict_h25944

Different levels of data:
  1. PHD brief
  2. PHD normal







AA : amino acid sequence
PHD_sec: PHD predicted secondary structure: H=helix, E=extended (sheet), blank=other (loop)
PHD = PHD: Profile network prediction HeiDelberg
Rel_sec: reliability index for PHDsec prediction (0=low to 9=high)
Note: for the brief presentation strong predictions marked by '*'
SUB_sec: subset of the PHDsec prediction, for all residues with an expected average accuracy > 82% (tables in header)
NOTE: for this subset the following symbols are used:
L: is loop (for which above ' ' is used)
.: means that no prediction is made for this residue, as the reliability is: Rel < 5
pH_sec: 'probability' for assigning helix (1=high, 0=low)
pE_sec: 'probability' for assigning strand (1=high, 0=low)
pL_sec: 'probability' for assigning neither helix, nor strand (1=high, 0=low)




PHD results (brief)

....,....1....,....2....,....3....,....4....,....5....,....6 AA RLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQWEDPSQASKVCQRL PHD_sec EEEEE EEEE EEEEEEE EEEEEE HHHHHH HHHHHHHHHH Rel_sec * *** ** **** ******* ****** * ***** *** * ******* ....,....11...,....12...,....13...,....14...,....15 AA NCGVPLSLGPFLVTYTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLE PHD_sec EEE EEEEEE EE EEEE Rel_sec **** *** *** ***** **** *********** ** *


PHD results (normal)

....,....1....,....2....,....3....,....4....,....5....,....6 AA RLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQWEDPSQASKVCQRL PHD_sec EEEEE EEEE EEEEEEE EEEEEE HHHHHH HHHHHHHHHH Rel_sec 905673682798616998786289999517477897423121347860614699998543 SUB_sec L.EEE.LL.EEEE.LLLLLLL.EEEEEE.L.EEEEE........HHH.L..HHHHHHH.. ....,....11...,....12...,....13...,....14...,....15 AA NCGVPLSLGPFLVTYTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLE PHD_sec EEE EEEEEE EE EEEE Rel_sec 77883432586334598459998165553113788899875774145639 SUB_sec LLLL....LLL...LLL.EEEEE.LLLL....LLLLLLLLLLL...EE.L



END of results for file predict_h25944





Quotes for methods

  1. PredictProtein: PredicProtein: B Rost (1996) Methods in Enzymology, 266:525-539
  2. ProSite: K Hofmann , P Bucher, L Falquet, A Bairoch:: The PROSITE database, its status in 1999. Nucleic Acids Res, 27, 215-219, 1999
  3. ProDom: F Corpet, F Servant, J Gouzy, and D Kahn:: ProDom and ProDom-CG: tools for protein domain analysis and whole genome comparisons. Nucleic Acids Res, 28, 267-269, 2000
  4. PHD: B Rost:: PHD: predicting one-dimensional protein structure by profile based neural networks. Methods in Enzymology, 266, 525-539, 1996
  5. PHDsec: B Rost & C Sander (1993) J. of Molecular Biology, 232:584-599




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