Ejemplo

Science
Submitted on November 20, 2003 Accepted on February 20, 2004
Environmental Genome Shotgun Sequencing of the Sargasso Sea
J. Craig Venter 1*, Karin Remington 1, John F. Heidelberg 2, Aaron L. Halpern 3, Doug Rusch 3, Jonathan A. Eisen 2, Dongying Wu 2, Ian Paulsen 2, Karen E. Nelson 2, William Nelson 2, Derrick E. Fouts 2, Samuel Levy 3, Anthony H. Knap 4, Michael W. Lomas 4, Ken Nealson 5, Owen White 2, Jeremy Peterson 2, Jeff Hoffman 1, Rachel Parsons 4, Holly Baden-Tillson 1, Cynthia Pfannkoch 1, Yu-Hui Rogers 6, Hamilton O. Smith 1
We have applied "whole genome shotgun sequencing" to microbial populations collected en mass on tangential flow and impact filters from sea water samples collected from the Sargasso Sea near Bermuda. A total of 1.045 billion basepairs of non-redundant sequence was generated, annotated and analyzed to elucidate the gene content, and diversity and relative abundance of the organisms within these environmental samples. These data are estimated to derive from at least 1800 genomic species based on sequence relatedness, including 148 novel bacterial phylotypes. We have identified over 1.2 million new genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity.

Science