(Planning
of the week)
Week is
colour coded in to Theory, Practical and theory & Breaks
The attendees will be divided
into Group 1 and Group 2 on Tuesday and
Wednesday
Assistants during the
practical classes are:
Willy Bienvenut - WB
Jennifer Burgess - JEB
Garry Corthals - GC
Carla Pasquarello - CAP
Manfredo Quadroni - MQ
Alexander Scherl - ALS
Monday
|
Day 1 WB MQ GC CAP |
9:30 |
Welcome Coffee |
10:00 |
Introduction: Perspective of
the week and scope of the course WB MQ GC |
11:00 |
Excision of spots from
acrylamide gels; start protein reduction WB CAP |
12:00 |
Lunch |
13:30 |
Protein alkylation WB CAP |
14:00 |
Protocol discussion: in-gel
digestion CAP |
14:30 |
Washing and destaining of CBB/SS
spots WB CAP |
15:30 |
Coffee break |
15:45 |
Introduction to Electrospray
and MALDI MS MQ |
16:30 |
Start trypsin digestion WB CAP |
17:00 |
Seminar by Dr Anne Estreicher
(SWISS-PROT) group: “Swiss-Prot
and other databases, and other things
perhaps” |
Tuesday |
Day 2 GROUP 1 CAP WB GC |
8:30 |
Introduction- program of the day |
9:00 |
Peptide extraction
after digestion and deposition on MALDI plate CAP WB GC |
9:45 |
Coffee break |
10:00 |
Sample deposition on
MALDI plate and MALDI preparation CAP WB GC |
12:00 |
Lunch |
13:30 |
Packing of micro-HPLC
columns GC WB |
14:30 |
MALDI-TOF analysis
in detail |
15:15
|
Coffee break |
15:30
|
MALDI-TOF analysis of
samples CAP WB GC |
17:00 |
Seminar by Dr
Jean-Charles Sanchez: “Applications in biomedical proteomics” |
Tuesday |
Day 2 GROUP 2 JEB ALS MQ |
8:30 |
Introduction- program of the day |
9:00 |
Peptide extraction;
set-up HPLC: solvents, gradients JEB ALS MQ |
9:45
|
Coffee break |
10:00 |
LC-MS: short and long
gradients; start samples JEB ALS MQ |
12:00 |
Lunch |
13:30 |
LC-MS: short and long
gradients JEB ALS MQ |
14:30 |
LC-MS/MS analysis in
detail MQ |
15:15 |
Coffee break |
15:30 |
LC-MS: short and long
gradients JEB ALS MQ |
17:00 |
Seminar by Dr
Jean-Charles Sanchez: “Applications in biomedical proteomics” |
Wednesday |
Day 3 GROUP 2 CAP WB GC |
8:30 |
Introduction- program of the day |
9:00 |
Peptide extraction
after digestion and deposition on MALDI plate CAP WB GC |
9:45 |
Coffee break |
10:00 |
Sample deposition on
MALDI plate and MALDI preparation CAP WB GC |
12:00 |
Lunch Seminar by Dr
Manfredo Quadroni: “Chemical protein modification for advance identification” |
13:30 |
Packing of micro-HPLC
columns GC WB |
14:30 |
MALDI-TOF analysis
in detail |
15:15
|
Coffee break |
15:30
|
MALDI-TOF analysis of
samples CAP WB GC |
17:00 |
Tutorial: de novo sequencing |
Wednesday |
Day 3 GROUP 1 JEB ALS MQ |
8:30 |
Introduction- program of the day |
9:00 |
Peptide extraction;
set-up HPLC: solvents, gradients JEB ALS MQ |
9:45
|
Coffee break |
10:00 |
LC-MS: short and long
gradients; start samples JEB ALS MQ |
12:00 |
Lunch Seminar by Dr
Manfredo Quadroni: “Chemical protein modification for advance identification” |
13:30 |
LC-MS: short and long
gradients JEB ALS MQ |
14:30 |
LC-MS/MS analysis in
detail MQ |
15:15 |
Coffee break |
15:30 |
LC-MS: short and long
gradients JEB ALS MQ |
17:00 |
Tutorial: de novo sequencing |
Thursday |
Day 4 CAP JEB WB ALS MQ GC |
8:30 |
Introduction-
program of the day |
9:00 |
MALDI tools or MSMS software tools CAP JEB WB ALS MQ GC |
9:45 |
Coffee break |
10:00 |
MALDI: data evaluation and analysis WB |
12:00 |
Lunch |
13:30 |
MALDI tools or MSMS software tools CAP JEB WB ALS MQ GC |
14:30 |
LC-MS/MS data evaluation and analysis in
detail MQ GC |
15:15 |
Coffee break |
15:30 |
LC-MS/MS data evaluation and analysis in
detail MQ GC |
17:00 |
Seminar by Dr Garry Corthals: “Strategies for
determination of phosphorylation” |
Friday |
Day 5 MQ GC CAP |
8:30 |
Introduction- program of the day |
9:00 |
De novo sequencing excesise Theory: instruments
and applications MQ GC |
9:45 |
Coffee break |
10:00 |
MS case studies |
12:00 |
Lunch |
13:30 |
roundtable discussion
of all experiments AMAP |
15:15
|
Coffee break |
15:30
|
roundtable discussion
of all experiments AMAP |
17:00
|
Beer o’clock AMAP |
ExPASy Home page | Site Map | Search ExPASy | Contact us |
Hosted by NCSC US | Mirror sites: | Canada | China | Korea | Switzerland | Taiwan |