Proteomics Profiling Training Course
Geneva
Proteomics Centre
(Planning of the week September 29th – October 3rd )
Week is color coded in to Theory, Practical and theory & Breaks
Day 1 : Monday
10:00 – 11:00 Welcome
and Introduction by Dr. Jean-Charles Sanchez and Dr. Odile Carrette
‘Perspective of the week and scope of the course’
11:00
– 12:00 Dr. Odile Carrette ‘SELDI Introduction’
12:00 – 14:00 LUNCH
13:30 – 15:00 SELDI –
SAX Profiling – Rententate Chromatography on Chip
15:00 – 16:30 SELDI –
ProteinChip Reader Software
16:30 – 17:00 Discussion meeting with trainers
17:00 – 18:00 Dr. Willy
Bienvenut ‘Quantitation by chemical modification’
Day 2 :
Tuesday
9:00 – 11:00 LC-
Prefractionation with SAX spin column
11:00
– 12:00 1DE Tricine Gels
12 :00
– 14 :00 LUNCH
14:00 – 16:00 SELDI -
analysis of the fractions
16:00 – 16:30 Discussion meeting with the
trainers
16:30 – 17:00 1DE
Tricine gels staining
17:00 – 18:00 Dr. Garry
Corthals ‘Introduction to Protein identification by MS’
Day 3:
Wednesday
9:00 – 10:30 1DE
Tricine gel staining and scanning
10:30 – 11:00 1DE -
Excision of the Interesting bands for MS
11 :00
– 12 :00 2DE
– Dr. Jean-Charles Sanchez ‘2DE sample preparation’
12 :00
– 14 :00 LUNCH
14 :00 –
15 :00 2DE – IPG strip rehydratation
15:00 – 16:30 2DE – Gel
Casting
16:30 – 17:00 Discussion meeting with trainers
17:00 -. 18:00 Dr Jean-Charles Sanchez ‘Applications in biomedical proteomics’
Day 4 :
Thursday
9:00
– 10:00 2DE – First dimension
10 :00
– 11 :00 2DE – Image analysis software : Melanie 4.0
11 :00 –
12 :00 2DE – Second dimension and staining
12:00 – 14:00 LUNCH
14:00- 16:00 2DE Gel
staining
16:00 – 16:30 2DE Gel
scanning
16:30 – 17:00 Discussion meeting with trainers
17:00 – 18:00 Dr.
Pierre-Alain Binz ‘Bioinformatics and Proteomics’
Day 5 : Friday
9:00
– 11:00 Dr. Sonja Voordijk ‘Image analysis software: MELANIE 4.0’
11:00 – 12:00 Dr.
Manfredo Quadroni ‘Sample Preparation for MS’
12:00 – 13:30 LUNCH
13:30 – 15:30 Discussion: Back to your lab, what
to do with your own project…
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