Proteomics Profiling Training Course

 

Geneva Proteomics Centre

University of Geneva

 

(Planning of the week September 29th – October  3rd )

 

Week is color coded in to Theory, Practical and theory & Breaks

 

Day 1 : Monday

 


10:00 – 11:00              Welcome and Introduction by Dr. Jean-Charles Sanchez and Dr. Odile Carrette ‘Perspective of the week and scope of the course’

 

11:00 – 12:00              Dr. Odile Carrette ‘SELDI Introduction’

 

12:00 – 14:00              LUNCH

 

13:30 – 15:00              SELDI – SAX Profiling – Rententate Chromatography on Chip

 

15:00 – 16:30              SELDI – ProteinChip Reader Software

 

16:30 – 17:00              Discussion meeting with trainers

 

17:00 – 18:00              Dr. Willy Bienvenut ‘Quantitation by chemical modification’           

 

 

Day 2 : Tuesday

 


9:00 – 11:00                LC- Prefractionation with SAX spin column

 

11:00 – 12:00              1DE Tricine Gels

 

12 :00 – 14 :00            LUNCH

 

14:00 – 16:00              SELDI - analysis of the fractions

 

16:00 – 16:30              Discussion meeting with the trainers

 

16:30 – 17:00              1DE Tricine gels staining

 

17:00 – 18:00              Dr. Garry Corthals ‘Introduction to Protein identification by MS’

 

 

 

 

 

 

 

 

Day 3: Wednesday

 


9:00 – 10:30                1DE Tricine gel staining and scanning

 

10:30 – 11:00              1DE - Excision of the Interesting bands for MS

 

11 :00 – 12 :00            2DE – Dr. Jean-Charles Sanchez ‘2DE sample preparation’

 

12 :00 – 14 :00            LUNCH

 

14 :00 – 15 :00            2DE – IPG strip rehydratation

 

15:00 – 16:30              2DE – Gel Casting

 

16:30 – 17:00              Discussion meeting with trainers

 

17:00 -. 18:00             Dr Jean-Charles Sanchez ‘Applications in biomedical proteomics’

 

 

Day 4 : Thursday

 


9:00 – 10:00                2DE – First dimension

 

10 :00 – 11 :00            2DE – Image analysis software : Melanie 4.0

 

11 :00 – 12 :00            2DE – Second dimension and staining

 

12:00 – 14:00              LUNCH

 

14:00- 16:00               2DE Gel staining

 

16:00 – 16:30              2DE Gel scanning

 

16:30 – 17:00              Discussion meeting with trainers

 

17:00 – 18:00              Dr. Pierre-Alain Binz ‘Bioinformatics and Proteomics’

 

 

 

Day 5 : Friday

 


9:00 – 11:00                Dr. Sonja Voordijk ‘Image analysis software: MELANIE 4.0’

 

11:00 – 12:00              Dr. Manfredo Quadroni ‘Sample Preparation for MS’

 

12:00 – 13:30              LUNCH

 

13:30 – 15:30              Discussion: Back to your lab, what to do with your own project…

 

 

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