Fig 4A. Output of the TagIdent tool where it was used to list all proteins
in a specified pI and Mw region, for one or more species. This approach is
useful to show proteins in the database that may correspond to the identity
of a protein on a 2-D gel. Here we enquired as to the possible identity of
a protein of approximate mass 9000 and of pI 6.5 from a protozoan. The
protein ubiquitin was found to be present in many protozoa in this pI and Mw
region. Whilst ubiquitin was the only protein present in the output here,
the identity of the protein as ubiquitin would need to be confirmed by
analytical techniques.
Note that this approach can also be used where the mass of an entire protein has
been accurately determined by mass spectrometry. In such a case, the mass
window used for searching can be quite small (e.g. mass ± 0.5%).
---------------------
Query name: CH8055
Search performed with following values:
pI = 6.5 Mw = 9000
delta-pI = 0.25 delta-Mw = 1800
OS or OC = PROTOZOA
---------------------
8 proteins found
UBIQ_ACACA (P49634)
pI: 6.56, MW: 8595.81
UBIQUITIN.
UBIQ_DICDI (P08618)
pI: 6.56, MW: 8537.73
UBIQUITIN.
UBIQ_EIMBO (P46574)
pI: 6.56, MW: 8550.75
UBIQUITIN.
UBIQ_EUPEU (P23324)
pI: 6.55, MW: 8581.77
UBIQUITIN.
UBIQ_LEIMA (Q05550)
pI: 6.57, MW: 8548.78
UBIQUITIN.
UBIQ_TETPY (P20685)
pI: 6.56, MW: 8524.72
UBIQUITIN.
UBIQ_TRYBB (P15174)
pI: 6.57, MW: 8506.74
UBIQUITIN.
UBIQ_TRYCR (P08565)
pI: 6.56, MW: 8508.72
UBIQUITIN.
-------------------
Last modified 16/Feb/1999 by CHH
ExPASy Home page