Fig 4B: Output of the TagIdent tool where it was used for protein
identification. A protein from an E. coli 2-D gel was uniquely identified by
virtue of its N-terminal sequence tag, estimated pI and mass. Whilst the
program was requested here to display protein N-termini, it will show any
protein that carries a specified tag in the "results with tagging" list, be
it found at a protein N-terminus, C-terminus or internally. Here the
identification of the protein as DHE4_ECOLI is convincing not only because
the tag is at the amino terminus, but because the tag was not found anywhere
in the sequence of the other 331 proteins also within the specified pI and
Mw window.
---------------------
Search performed with following values:
pI = 5.97 Mw = 45098
delta-pI = 0.50 delta-Mw = 9019
OS or OC = ESCHERICHIA
Sequence Tag = MDQT
Display the N-terminal sequence.
Print only the sequences matching your tag MDQT.
---------------------
332 proteins found
---
Results with tagging: 1 found
The number before the sequence indicates the position in the
sequence where your tag MDQT has been found (first occurrence).
The sequence tag itself is printed in lowercase.
---
DHE4_ECOLI (P00370)
NADP-SPECIFIC GLUTAMATE DEHYDROGENASE (EC 1.4.1.4).
pI: 5.98, MW: 48581.14
1 mdqtYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLE...
---
Results without tagging: 331 found
---------------------
Last modified 17/Aug/1999 by ELG
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