GENSCAN 1.0 Date run: 5-Apr-102 Time: 05:55:59 Sequence human : 2481 bp : 54.86% C+G : Isochore 3 (51 - 57 C+G%) Parameter matrix: HumanIso.smat Predicted genes/exons: Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr.. ----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------ 1.01 Init + 739 1038 300 0 0 90 115 426 0.733 42.75 1.02 Term + 1146 1205 60 2 0 99 41 72 0.615 1.70 1.03 PlyA + 2206 2211 6 1.05Click here to view a PDF image of the predicted gene(s)
Click here for a PostScript image of the predicted gene(s)
Predicted peptide sequence(s):
Predicted coding sequence(s):
>human|GENSCAN_predicted_peptide_1|119_aa
MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAEEPLSLLDDMNHCYSRLRELVPGVPR
GTQLSQVEILQRVIDYILDLQVVLAEPAPGPPDGPHLPIQTAELAPELVISNDKRSFCH
>human|GENSCAN_predicted_CDS_1|360_bp
atgaaggcgctgagcccggtgcgcggctgctacgaggcggtgtgctgcctgtcggaacgc
agtctggccatcgcccggggccgagggaagggcccggcagctgaggagccgctgagcttg
ctggacgacatgaaccactgctactcccgcctgcgggaactggtacccggagtcccgaga
ggcactcagcttagccaggtggaaatcctacagcgcgtcatcgactacattctcgacctg
caggtagtcctggccgagccagcccctggaccccctgatggcccccaccttcccatccag
acagccgagctcgctccggaacttgtcatctccaacgacaaaaggagcttttgccactga
Explanation
Gn.Ex : gene number, exon number (for reference)
Type : Init = Initial exon (ATG to 5' splice site)
Intr = Internal exon (3' splice site to 5' splice site)
Term = Terminal exon (3' splice site to stop codon)
Sngl = Single-exon gene (ATG to stop)
Prom = Promoter (TATA box / initation site)
PlyA = poly-A signal (consensus: AATAAA)
S : DNA strand (+ = input strand; - = opposite strand)
Begin : beginning of exon or signal (numbered on input strand)
End : end point of exon or signal (numbered on input strand)
Len : length of exon or signal (bp)
Fr : reading frame (a forward strand codon ending at x has frame x mod 3)
Ph : net phase of exon (exon length modulo 3)
I/Ac : initiation signal or 3' splice site score (tenth bit units)
Do/T : 5' splice site or termination signal score (tenth bit units)
CodRg : coding region score (tenth bit units)
P : probability of exon (sum over all parses containing exon)
Tscr : exon score (depends on length, I/Ac, Do/T and CodRg scores)
Comments
The SCORE of a predicted feature (e.g., exon or splice site) is a
log-odds measure of the quality of the feature based on local sequence
properties. For example, a predicted 5' splice site with
score > 100 is strong; 50-100 is moderate; 0-50 is weak; and
below 0 is poor (more than likely not a real donor site).
The PROBABILITY of a predicted exon is the estimated probability under
GENSCAN's model of genomic sequence structure that the exon is correct.
This probability depends in general on global as well as local sequence
properties, e.g., it depends on how well the exon fits with neighboring
exons. It has been shown that predicted exons with higher probabilities
are more likely to be correct than those with lower probabilities.