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Northeast Structural Genomics Consortium

Gaetano T. Montelione, Ph.D. Rutgers University
Burkhard Rost t, Ph.D. Columbia University, NY
Stephen Anderson, Ph.D. Rutgers University
Cheryl Arrowsmith, Ph.D. University of Toronto and Ontario Cancer Institute
George T. DeTitta, Ph.D. Hauptman-Woodward Medical Research, Buffalo, NY
Aled Edwards, Ph.D. University of Toronto and
Mark Gerstein, Ph.D. Molecular Biophysics and Biochemistry
Wayne Hendrickson, Ph.D. Howard Hughes Medical Institute
Barry Honig, Ph.D. Biochemistry and Molecular Biophysics
John Hunt, Ph.D. Columbia University, NY, NY
Michael A. Kennedy, Ph.D. Pacific Northwest National Laboratory Richland, WA
Lynne Regan, Ph.D. Yale University, New Haven, CT
Thomas Szyperski, Ph.D. State University of New York at Buffalo Buffalo, NY
Liang Tong, Ph.D. Columbia University, NY
Hao Wu, Ph.D. Weill Medical College Cornell University, NY
Integrated technologies for high-throughput (htp) protein production and 3D structure determination.
i) bioinformatics methods for clustering and prioritizing candidate proteins for biophysical analysis,
ii) biotechnologies and robotic methods for htp expression plasmid construction, expression screening, and protein production,
iii) htp methods for analysis of "foldedness" of expressed proteins by biophysical techniques .
iv) cost efficient production of 13C, 15N, 2H, and/or SeMet enriched protein samples
v) experimental and theoretical methods for parsing large multidomain proteins into domain encoding segments,
vi) robotic methods for htp protein crystallization screening, solubility screening, and crystal manipulation,
vii) improved methods for rapid crystallographic data collection using synchrotron sources,
viii) new NMR pulse sequences and hardware providing rapid collection of data suitable for high-resolution 3D structures
ix) automated computational methods for determining 3D protein structures from raw diffraction and/or NMR data,
x) methods for analyzing 3D protein structures in order to develop testable hypotheses regarding biochemical functions,
xi) development of integrated project databases to keep track of the reagents and information generated
xii) organized dissemination of the results

Northeast Structural Genomics Consortium

Gaetano T. Montelione, Ph.D. Rutgers University

Burkhard Rost t, Ph.D. Columbia University, NY

Stephen Anderson, Ph.D. Rutgers University

Cheryl Arrowsmith, Ph.D. University of Toronto and Ontario Cancer Institute

George T. DeTitta, Ph.D. Hauptman-Woodward Medical Research, Buffalo, NY

Aled Edwards, Ph.D. University of Toronto and

Mark Gerstein, Ph.D. Molecular Biophysics and Biochemistry

Wayne Hendrickson, Ph.D. Howard Hughes Medical Institute

Barry Honig, Ph.D. Biochemistry and Molecular Biophysics

John Hunt, Ph.D. Columbia University, NY, NY

Michael A. Kennedy, Ph.D. Pacific Northwest National Laboratory Richland, WA

Lynne Regan, Ph.D. Yale University, New Haven, CT

Thomas Szyperski, Ph.D. State University of New York at Buffalo Buffalo, NY

Liang Tong, Ph.D. Columbia University, NY

Hao Wu, Ph.D. Weill Medical College Cornell University, NY

Integrated technologies for high-throughput (htp) protein production and 3D structure determination.

i) bioinformatics methods for clustering and prioritizing candidate proteins for biophysical analysis,

ii) biotechnologies and robotic methods for htp expression plasmid construction, expression screening, and protein production,

iii) htp methods for analysis of "foldedness" of expressed proteins by biophysical techniques .

iv) cost efficient production of 13C, 15N, 2H, and/or SeMet enriched protein samples

v) experimental and theoretical methods for parsing large multidomain proteins into domain encoding segments,

vi) robotic methods for htp protein crystallization screening, solubility screening, and crystal manipulation,

vii) improved methods for rapid crystallographic data collection using synchrotron sources,

viii) new NMR pulse sequences and hardware providing rapid collection of data suitable for high-resolution 3D structures

ix) automated computational methods for determining 3D protein structures from raw diffraction and/or NMR data,

x) methods for analyzing 3D protein structures in order to develop testable hypotheses regarding biochemical functions,

xi) development of integrated project databases to keep track of the reagents and information generated

xii) organized dissemination of the results