BLASTP2 Result
of 
Bork Group's Advanced BLAST2 Search Service at 
EMBL.
back to BLAST2
BLASTP 2.0MP-WashU [16-Dec-1999] [irix6-r10k-L64 23:35:48 16-Dec-1999]
Copyright (C) 1996-1999 Washington University, Saint Louis, Missouri USA.
All Rights Reserved.
Reference: Gish, W. (1996-1999) http://blast.wustl.edu
Query= query_sequence
(260 letters)
Echofilter:
>Filtered+0
ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGG
EKAFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCD
IIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS
KIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATD
DRREGMSAFVEKRKANFKDH
Database: nrdb-95%
882,049 sequences; 286,239,830 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done
Query sequence and locally-aligned HSPs from database sequences
0 60 120 180 240
| | | | | |260
query_sequence | __________________________________________________
^ v swissnew|P14604|EC | __________________________________________________
^ v trembl|BT008161|BT | __________________________________________________
^ v sptrembl|Q95KZ6|Q9 | __________________________________________________
^ v sptrembl|Q7ZZ04|Q7 | __________________________________________________
^ v sptrembl|Q9V6U5|Q9 | __________________________________________________
^ v swiss|P34559|ECHM_ | _________________________________________________
^ v sptrembl|Q8YED3|Q8 | __________________________________________________
^ v sptrembl|Q98BG7|Q9 | _________________________________________________
^ v sptrembl|Q8UIH3|Q8 | __________________________________________________
^ v swiss|Q52995|ECHH_ | __________________________________________________
^ v sptrembl|Q9NEZ8|Q9 | ________________________________________________
^ v sptrembl|Q89QT8|Q8 | _________________________________________________
^ v sptremblnew|CAE272 | __________________________________________________
^ v sptrembl|Q8XVF9|Q8 | __________________________________________________
^ v sptrembl|Q9AHX8|Q9 | __________________________________________________
^ v sptrembl|Q9AC55|Q9 | __________________________________________________
^ v sptrembl|Q880J4|Q8 | __________________________________________________
^ v sptrembl|Q7VU52|Q7 | _________________________________________________
^ v tremblnew|AE017230 | _________________________________________________
^ v swissnew|O53418|EC | _________________________________________________
^ v swiss|O07137|ECH8_ | ______________________________________________
^ v sptrembl|O84978|O8 | ______________________________________________
^ v sptremblnew|CAE451 | _____________________________________________
^ v sptrembl|Q88HR9|Q8 | ______________________________________________
^ v sptrembl|Q9Z9V2|Q9 | ______________________________________________
^ v sptrembl|Q845K2|Q8 | ______________________________________________
^ v swiss|P76082|PAAF_ | _______________________________________________
^ v sptrembl|O07453|O0 | _________________________________________________
^ v sptrembl|Q96XV1|Q9 | ______________________________________________
^ v sptrembl|Q8RC92|Q8 | ______________________________________________
^ v sptrembl|Q891F2|Q8 | __________________________________________________
^ v sptrembl|P97087|P9 | ______________________________________________
^ v sptrembl|Q97VK0|Q9 | ______________________________________________
^ v sptrembl|Q8XI23|Q8 | _________________________________________________
^ v sptrembl|Q98LI4|Q9 | ______________________________________________
^ v sptrembl|Q970T6|Q9 | _______________________________________________
^ v sptrembl|Q8BU95|Q8 | _____________________
^ v sptremblnew|AAR347 | _______________________________________________
^ v sptrembl|Q9I393|Q9 | ______________________________________________
^ v sptrembl|O29299|O2 | _____________________________________________
^ v tremblnew|BX682953 | _________________________________________________
^ v tremblnew|BX842651 | __________________________________________________
^ v sptrembl|O29814|O2 | ______________________________________________
^ v sptrembl|Q9F7E7|Q9 | _______________________________________________
^ v sptrembl|Q8XP70|Q8 | _______________________________________________
^ v sptrembl|Q937T3|Q9 | ______________________________________________
^ v sptrembl|Q898H3|Q8 | _______________________________________________
^ v sptrembl|Q8RMI7|Q8 | _________________________________________________
^ v swiss|P52046|CRT_C | _________________________________________________
^ v sptrembl|Q9YBW6|Q9 | ______________________________________________
Alignments:
WARNING: Descriptions of 751 database sequences were not reported due to the
limiting value of parameter V = 50.
^ = swissnew|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precursor (EC
4.2.1.17) (Short chain enoyl-CoA hydratase) (SCEH) (Enoyl-CoA
hydratase 1).//:swiss|P14604|ECHM_RAT Enoyl-CoA hydratase,
mitochondrial precursor (EC 4.2.1.17) (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase
1).//:sptremblnew|AAH64655|AAH64655 Hypothetical
protein.//:tremblnew|BC064655|BC064655_1 product: "Unknown (protein
for MGC:72336)"; Rattus norvegicus cDNA clone MGC:72336
IMAGE:5599860, complete cds. //:trembl|X15958|RNECH_1 product:
"precursor polypeptide (AA -29 to 261)"; Rat mRNA for
mitochondrial enoyl-CoA hydratase (EC 4.2.1.17) //:gp|X15958|56072
precursor polypeptide (AA -29 to 261) [Rattus norvegicus]
Length = 290
0 60 120 180 240
| | | | | |290
swissnew|P14604|ECHM_ | __________________________________________________
Local hits (HSPs) | ____________________________________________
Score = 1312 (466.9 bits), Expect = 1.6e-133, P = 1.6e-133
Identities = 259/260 (99%), Positives = 259/260 (99%)
Query: 1 ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGG 60
ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGG
Sbjct: 31 ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGG 90
Query: 61 EKAFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCD 120
EKAFAAG DIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCD
Sbjct: 91 EKAFAAGADIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCD 150
Query: 121 IIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS 180
IIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS
Sbjct: 151 IIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS 210
Query: 181 KIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATD 240
KIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATD
Sbjct: 211 KIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATD 270
Query: 241 DRREGMSAFVEKRKANFKDH 260
DRREGMSAFVEKRKANFKDH
Sbjct: 271 DRREGMSAFVEKRKANFKDH 290
^ = trembl|BT008161|BT008161_1 product: "Homo sapiens enoyl Coenzyme A
hydratase, short chain, 1, mitochondrial"; Synthetic construct
Homo sapiens enoyl Coenzyme A hydratase, short chain, 1,
mitochondrial mRNA, partial cds. //:gp|BT008161|30585161 Homo
sapiens enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
[synthetic construct]
Length = 291
0 60 120 180 240
| | | | | |291
trembl|BT008161|BT008 | __________________________________________________
Local hits (HSPs) | ____________________________________________
Score = 1201 (427.8 bits), Expect = 9.5e-122, P = 9.5e-122
Identities = 232/259 (89%), Positives = 250/259 (96%)
Query: 1 ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGG 60
ANF+YII EK+GKN++VGLIQLNRPKALNALC+GLI+ELNQAL+ FEEDPAVGAIVLTGG
Sbjct: 31 ANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGG 90
Query: 61 EKAFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCD 120
+KAFAAG DIKEMQN +FQDCYS KFL HWDH+T++KKPVIAAVNGYA GGGCELAMMCD
Sbjct: 91 DKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCD 150
Query: 121 IIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS 180
IIYAGEKAQF QPEIL+GTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS
Sbjct: 151 IIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS 210
Query: 181 KIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATD 240
KI PVETLVEEAIQCAEKIA+NSKI+VAMAKESVNAAFEMTLTEG+KLEKKLFYSTFATD
Sbjct: 211 KICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATD 270
Query: 241 DRREGMSAFVEKRKANFKD 259
DR+EGM+AFVEKRKANFKD
Sbjct: 271 DRKEGMTAFVEKRKANFKD 289
^ = sptrembl|Q95KZ6|Q95KZ6 Enoyl-CoA hydratase precursor (EC 4.2.1.17)
(Fragment).//:trembl|AY049961|AY049961_1 gene: "ECH"; product:
"enoyl-CoA hydratase precursor"; Bos taurus enoyl-CoA hydratase
precursor (ECH) mRNA, partial cds; nuclear gene for mitochondrial
product. //:gp|AY049961|15982640 enoyl-CoA hydratase precursor [Bos
taurus]
Length = 264
0 60 120 180 240
| | | | | |264
sptrembl|Q95KZ6|Q95KZ | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 1150 (409.9 bits), Expect = 2.4e-116, P = 2.4e-116
Identities = 227/259 (87%), Positives = 240/259 (92%)
Query: 1 ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGG 60
A F+YIIT KKG+NS+VGLIQLNRPKALNALCNGLI ELNQAL+ FEEDPAVGAIVLTGG
Sbjct: 5 AAFEYIITAKKGRNSNVGLIQLNRPKALNALCNGLIVELNQALQAFEEDPAVGAIVLTGG 64
Query: 61 EKAFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCD 120
EK FAAG DIKEMQ+ TFQ+CYSG FLSHWD +TR+KKPVIAAVNGYALGGGCELAMMCD
Sbjct: 65 EKVFAAGADIKEMQSLTFQNCYSGGFLSHWDQLTRVKKPVIAAVNGYALGGGCELAMMCD 124
Query: 121 IIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS 180
IIYAGEKAQFGQPEIL+GTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS
Sbjct: 125 IIYAGEKAQFGQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS 184
Query: 181 KIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATD 240
KIFPVET+VEEAIQCAEKIA+NSKI+ AMAKESVNAAFEMTL EG KLEKKLFYSTFA
Sbjct: 185 KIFPVETVVEEAIQCAEKIASNSKIVTAMAKESVNAAFEMTLAEGVKLEKKLFYSTFAPK 244
Query: 241 DRREGMSAFVEKRKANFKD 259
R+ AFVEKRKANFKD
Sbjct: 245 TGRKAWPAFVEKRKANFKD 263
^ = sptrembl|Q7ZZ04|Q7ZZ04 SI:zC217G15.1 (Novel protein similar to vertebrate
mitochondrial enoyl coenzyme A hydratase 1 (ECHS1)
).//:trembl|AL831745|AL831745_1 gene: "SI:zC217G15.1"; product:
"SI:zC217G15.1 (novel protein similar to vertebrate mitochondrial
enoyl Coenzyme A hydratase 1 (ECHS1) )"; Zebrafish DNA sequence
from clone CH211-217G15 in linkage group 13 Contains a novel gene
similar to ECHS1 (vertebrate mitochondrial enoyl Coenzyme A
hydratase 1), four novel genes, the msxc gene for muscle segment
homeobox protein C and a novel gene for a protein similar to
reprolysin zinc metalloproteases. //:gp|AL831745|27802778
SI:zC217G15.1 (novel protein similar to vertebrate mitochondrial
enoyl Coenzyme A hydratase 1 (ECHS1) ) [Danio rerio]
Length = 291
0 60 120 180 240
| | | | | |291
sptrembl|Q7ZZ04|Q7ZZ0 | __________________________________________________
Local hits (HSPs) | ___________________________________________
Score = 1047 (373.6 bits), Expect = 2.0e-105, P = 2.0e-105
Identities = 198/257 (77%), Positives = 232/257 (90%)
Query: 3 FQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEK 62
++YI+ +KKG+ +VG IQLNRPKALNALC+GL+ E+ +AL+ FE D VGAIV+TG EK
Sbjct: 34 YEYILVDKKGEKKNVGFIQLNRPKALNALCDGLMLEVGKALDAFEMDSEVGAIVVTGSEK 93
Query: 63 AFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDII 122
AFAAG DIKEMQNRTFQ+CY G FL+HW+ ++ +KKPVIAAVNG+ALGGGCE AMMCDII
Sbjct: 94 AFAAGADIKEMQNRTFQECYGGNFLAHWNRVSTVKKPVIAAVNGFALGGGCEFAMMCDII 153
Query: 123 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 182
YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQ+AKQ+GLVSKI
Sbjct: 154 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQEAKQSGLVSKI 213
Query: 183 FPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDR 242
FPV+ LV EAI+C EKIA NSK++ AMAKESVNAAFE+TL EG++ EK+LF++TFAT+DR
Sbjct: 214 FPVDQLVPEAIKCGEKIAGNSKLVSAMAKESVNAAFELTLAEGSRFEKRLFHATFATEDR 273
Query: 243 REGMSAFVEKRKANFKD 259
+EGMSAFVEKRKA F+D
Sbjct: 274 KEGMSAFVEKRKAAFQD 290
^ = sptrembl|Q9V6U5|Q9V6U5 CG18645 protein
(LD24265p).//:trembl|AE003817|AE003817_24 product: "CG6543-PA";
"CG6543-PB"; Drosophila melanogaster chromosome 2R, section 33 of
74 of the complete sequence. //:trembl|BT003259|BT003259_1 gene:
"CG6543"; product: "LD24265p"; Drosophila melanogaster LD24265
full insert cDNA. //:gp|AE003817|7303265 CG6543-PA [Drosophila
melanogaster]//:gp|AE003817|7303266 CG6543-PB [Drosophila
melanogaster]//:gp|BT003259|27819957 LD24265p [Drosophila
melanogaster]
Length = 295
0 60 120 180 240
| | | | | |295
sptrembl|Q9V6U5|Q9V6U | __________________________________________________
Local hits (HSPs) | ___________________________________________
Score = 848 (303.6 bits), Expect = 2.4e-84, P = 2.4e-84
Identities = 161/256 (62%), Positives = 200/256 (78%)
Query: 2 NFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGE 61
N++YI TE G+ +VG+I LNRPKALNALCNGL++EL+ AL+ F +D + AIVLTG E
Sbjct: 37 NWEYIKTEVAGEGKNVGVITLNRPKALNALCNGLMKELSTALQQFSKDKTISAIVLTGSE 96
Query: 62 KAFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDI 121
KAFAAG DIKEM T+ C G FL+ W + R +KP+IAAVNGYALGGGCELAMMCDI
Sbjct: 97 KAFAAGADIKEMVGNTYSQCIQGNFLNDWTEVARTQKPIIAAVNGYALGGGCELAMMCDI 156
Query: 122 IYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSK 181
IYAG+KA+FGQPEI LGTIPGAGGTQRLTR VGKS AMEM LTG+ I AQ+A++ GL SK
Sbjct: 157 IYAGDKAKFGQPEIALGTIPGAGGTQRLTRVVGKSKAMEMCLTGNMIGAQEAEKLGLASK 216
Query: 182 IFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDD 241
+ P + L+ EA++ EKI +S +IV + KE+VN A+E TL EG K E++ F++TF+T D
Sbjct: 217 VVPADQLLGEAVKLGEKIGTHSNLIVQLCKEAVNTAYETTLQEGLKFERRTFHATFSTAD 276
Query: 242 RREGMSAFVEKRKANF 257
R+EGM+AF EKR A F
Sbjct: 277 RKEGMTAFAEKRPAKF 292
^ = swiss|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial (EC
4.2.1.17).//:tremblnew|Z27079|CET05G5_3 gene: "T05G5.6"; product:
"Hypothetical protein T05G5.6"; Caenorhabditis elegans cosmid
T05G5 //:trembl|Z27079|CET05G5_3 gene: "T05G5.6"; product:
"Hypothetical protein T05G5.6"; Caenorhabditis elegans cosmid
T05G5 //:gp|Z27079|3879490 Hypothetical protein T05G5.6
[Caenorhabditis elegans]
Length = 288
0 60 120 180 240
| | | | | |288
swiss|P34559|ECHM_CAE | __________________________________________________
Local hits (HSPs) | ____________________________________________
Score = 816 (292.3 bits), Expect = 6.0e-81, P = 6.0e-81
Identities = 160/255 (62%), Positives = 201/255 (78%)
Query: 4 QYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKA 63
+ I EK G+ +V LI+LNRPKALNALC L+ EL ALE + D +VGAIV+TG E+A
Sbjct: 32 EMIKIEKVGEKQNVALIKLNRPKALNALCAQLMTELADALEVLDTDKSVGAIVITGSERA 91
Query: 64 FAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIY 123
FAAG DIKEM N F +SG FLS+W ++ +KKPVIAAVNG+ALGGG ELAMMCDIIY
Sbjct: 92 FAAGADIKEMTNNEFATTFSGSFLSNWTAVSDVKKPVIAAVNGFALGGGNELAMMCDIIY 151
Query: 124 AGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIF 183
AGEKA+FGQPEI +GTIPGAGGTQR RA GKS AME+ LTG+ ++AQ+AK+ G+VSKIF
Sbjct: 152 AGEKARFGQPEINIGTIPGAGGTQRWARAAGKSFAMEVCLTGNHVTAQEAKEHGIVSKIF 211
Query: 184 PVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRR 243
P + +V EA++ EKIA+ S +IV MAKE+VN A+E+TL EG E++LF++TFAT DR+
Sbjct: 212 PADQVVGEAVKLGEKIADQSPLIVQMAKEAVNKAYELTLQEGLHFERRLFHATFATKDRK 271
Query: 244 EGMSAFVEKRKANFK 258
EGM+AF EKRK ++
Sbjct: 272 EGMTAFAEKRKPQWE 286
^ = sptrembl|Q8YED3|Q8YED3 Probable enoyl-CoA hydratase (EC
4.2.1.17).//:trembl|AE009628|AE009628_2 gene: "BMEI1945"; product:
"PROBABLE ENOYL-COA HYDRATASE"; Brucella melitensis 16M chromosome
I, section 185 of 195 of the complete sequence.
//:pironly|AC3495|AC3495 probable enoyl-CoA hydratase (EC 4.2.1.17)
[imported] - Brucella melitensis (str//:gp|AE009628|17983994
PROBABLE ENOYL-COA HYDRATASE [Brucella melitensis 16M]
Length = 293
0 60 120 180 240
| | | | | |293
sptrembl|Q8YED3|Q8YED | __________________________________________________
Local hits (HSPs) | ___________________________________________
Score = 751 (269.4 bits), Expect = 4.6e-74, P = 4.6e-74
Identities = 151/257 (58%), Positives = 193/257 (75%)
Query: 3 FQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEK 62
++ II E + SVGLI+LNRP+ALNAL ++EEL AL F+ D VGA+VLTG EK
Sbjct: 39 YETIIVETR---DSVGLIRLNRPQALNALNRAVLEELTDALAAFDADGKVGAVVLTGSEK 95
Query: 63 AFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDII 122
AFAAG DIKEMQ F D Y + W + RI+KP+IAAV+GYALGGGCELAMMCD I
Sbjct: 96 AFAAGADIKEMQLINFVDAYLQDMFADWQKVDRIRKPIIAAVSGYALGGGCELAMMCDFI 155
Query: 123 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 182
AG A+FGQPEI LG IPG GG+QRLTR VGKS AM+M LTG + A +A++ GLVS++
Sbjct: 156 IAGSNAKFGQPEITLGAIPGMGGSQRLTRYVGKSKAMDMCLTGRMMDAAEAERCGLVSRV 215
Query: 183 FPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDR 242
E L+EEA++ AE+IA+ S++ V MAKE+VN A+E TL EG + E++LF+S FAT+D+
Sbjct: 216 VAPEALLEEALKAAERIASFSRVSVYMAKEAVNRAYETTLDEGLRFERRLFHSLFATEDQ 275
Query: 243 REGMSAFVEKRKANFKD 259
+EGMSAFV+KR +FK+
Sbjct: 276 KEGMSAFVDKRTPSFKN 292
^ = sptrembl|Q98BG7|Q98BG7 Enoyl CoA hydratase.//:trembl|AP003007|AP003007_7
gene: "mll5584"; product: "enoyl CoA hydratase"; Mesorhizobium
loti DNA, complete genome, section 14/21. //:gp|AP003007|14025405
enoyl CoA hydratase [Mesorhizobium loti]
Length = 257
0 60 120 180 240
| | | | | |257
sptrembl|Q98BG7|Q98BG | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 738 (264.8 bits), Expect = 1.1e-72, P = 1.1e-72
Identities = 149/255 (58%), Positives = 191/255 (74%)
Query: 3 FQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEK 62
++ IITE +GK VGL+ LNRPKALNAL L+ EL A++ F+ D +GA+V+TG +K
Sbjct: 3 YETIITETRGK---VGLVTLNRPKALNALNLQLLAELVAAVKDFDADAGIGAVVITGSDK 59
Query: 63 AFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDII 122
AFAAG DIKEMQ +F D Y W+ +TR++KPVIAAV GYALGGGCELAMMCD I
Sbjct: 60 AFAAGADIKEMQAISFADAYLQDLFVGWEDLTRVRKPVIAAVAGYALGGGCELAMMCDFI 119
Query: 123 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 182
A + A+FGQPEI LG IPG GG+QRLTR VGKS AM+M LTG + A +A+++GLVS++
Sbjct: 120 IAADTAKFGQPEITLGVIPGMGGSQRLTRFVGKSKAMDMCLTGRMMDAAEAERSGLVSRV 179
Query: 183 FPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDR 242
P LVEEA++ A KIA+ S V MAKE+VN ++E TLTEG + E++LF+S FA +D+
Sbjct: 180 VPAAELVEEAVKAAAKIADFSLPSVMMAKEAVNRSYETTLTEGLRFERRLFHSLFALEDQ 239
Query: 243 REGMSAFVEKRKANF 257
+EGM+AF EKRK NF
Sbjct: 240 KEGMAAFAEKRKPNF 254
^ = sptrembl|Q8UIH3|Q8UIH3 Enoyl CoA hydratase.//:trembl|AE007970|AE007970_8
gene: "AGR_C_562"; product: "AGR_C_562p"; Agrobacterium
tumefaciens str. C58 circular chromosome, section 28 of 254 of the
complete sequence. //:pironly|A97398|A97398 probable enoyl-CoA
hydratase [imported] - Agrobacterium tumefaciens (strain
C58,//:gp|AE007970|15155225 AGR_C_562p [Agrobacterium tumefaciens
str. C58 (Cereon)]
Length = 288
0 60 120 180 240
| | | | | |288
sptrembl|Q8UIH3|Q8UIH | __________________________________________________
Local hits (HSPs) | ____________________________________________
Score = 738 (264.8 bits), Expect = 1.1e-72, P = 1.1e-72
Identities = 146/258 (56%), Positives = 193/258 (74%)
Query: 2 NFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGE 61
+++ I+ EK+ + VG+I LNRPK LNAL + L++EL L +F D ++GAI++TG E
Sbjct: 33 DYEMILVEKRDE---VGVITLNRPKVLNALNSVLLKELRHILSSFSADDSIGAIIITGSE 89
Query: 62 KAFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDI 121
KAFAAG DIKEMQ F D Y G FL WD + +KPVIAAV+G+ALGGGCELAMMCD
Sbjct: 90 KAFAAGADIKEMQGLDFVDAYVGDFLGGWDEVAATRKPVIAAVSGFALGGGCELAMMCDF 149
Query: 122 IYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSK 181
I A + A+FGQPEI LG IPG GG+QRLTRAVGK+ AM++VLTG + A +A++AGLVS+
Sbjct: 150 IIASDTAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLVLTGRMMDAAEAERAGLVSR 209
Query: 182 IFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDD 241
I P L+E A++ A +IA+ S+ V MAKESVN +FE+ L EG + E++ F S FAT+D
Sbjct: 210 IVPAADLMEVAVEAATRIASLSRASVLMAKESVNRSFEVPLAEGLRYERRAFQSLFATED 269
Query: 242 RREGMSAFVEKRKANFKD 259
++EGM+AFVEKRK F++
Sbjct: 270 QKEGMAAFVEKRKPAFRN 287
^ = swiss|Q52995|ECHH_RHIME Probable enoyl-CoA hydratase (EC
4.2.1.17).//:trembl|AL591783|SME591783_137 gene: "SMc01153";
product: "PROBABLE ENOYL COA HYDRATASE PROTEIN"; Sinorhizobium
meliloti 1021 complete chromosome; segment 2/12
//:gp|AL591783|15073295 PROBABLE ENOYL COA HYDRATASE PROTEIN
[Sinorhizobium meliloti]
Length = 257
0 60 120 180 240
| | | | | |257
swiss|Q52995|ECHH_RHI | __________________________________________________
Local hits (HSPs) | __________________________________________________
Score = 735 (263.8 bits), Expect = 2.3e-72, P = 2.3e-72
Identities = 148/257 (57%), Positives = 193/257 (75%)
Query: 2 NFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGE 61
+++ ++ E +G+ VGLI LNRP+ALNAL L+ EL+ AL+ F+ D AVGAIVL G E
Sbjct: 2 SYETLLVETQGR---VGLITLNRPQALNALNAVLMRELDAALKAFDADRAVGAIVLAGSE 58
Query: 62 KAFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDI 121
KAFAAG DIKEMQ F D Y FL W+H+ +KP+IAAV+G+ALGGGCELAMMCD
Sbjct: 59 KAFAAGADIKEMQGLDFVDGYLADFLGGWEHVANARKPMIAAVSGFALGGGCELAMMCDF 118
Query: 122 IYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSK 181
I A E A+FGQPEI LG IPG GG+QRLTRAVGK+ AM+++LTG + A +A+++GLVS+
Sbjct: 119 IIASETAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLILTGRMMDAAEAERSGLVSR 178
Query: 182 IFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDD 241
+ + L+EEA+ AEKIA+ S MAKE+VN + E+TL EG + E++LF S FAT+D
Sbjct: 179 VVAPDRLLEEALGAAEKIASFSLPAAMMAKEAVNRSLELTLAEGLRFERRLFQSLFATED 238
Query: 242 RREGMSAFVEKRKANFK 258
++EGM+AFV KRKA FK
Sbjct: 239 QKEGMAAFVAKRKAEFK 255
^ = sptrembl|Q9NEZ8|Q9NEZ8 Y105E8A.e protein.//:tremblnew|AL132876|CEY105E8A_2
gene: "Y105E8A.4"; product: "Hypothetical protein Y105E8A.4";
Caenorhabditis elegans YAC Y105E8A //:trembl|AL132876|CEY105E8A_2
gene: "Y105E8A.4"; product: "Hypothetical protein Y105E8A.4";
Caenorhabditis elegans YAC Y105E8A //:gp|AL132876|15130766
Hypothetical protein Y105E8A.4 [Caenorhabditis elegans]
Length = 256
0 60 120 180 240
| | | | | |256
sptrembl|Q9NEZ8|Q9NEZ | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 735 (263.8 bits), Expect = 2.3e-72, P = 2.3e-72
Identities = 146/248 (58%), Positives = 185/248 (74%)
Query: 6 IITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFA 65
I + G +V LI LNRP ALNALC L+ EL++ L E+D + IVLTG EKAFA
Sbjct: 2 ITLSRTGAAENVALITLNRPSALNALCRELMLELSENLLKVEKDQSYHVIVLTGSEKAFA 61
Query: 66 AGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAG 125
AG DIKEM F D + + ++WD ++ I KPVIAAVNG+ALGGG ELA+MCDI+YAG
Sbjct: 62 AGADIKEMAKLEFADVFENDYFTNWDTLSHITKPVIAAVNGFALGGGTELALMCDIVYAG 121
Query: 126 EKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPV 185
E A FGQPEI +GTIPG GGTQR R V KS+AME+ L+GDR+ AQ+AK+ GLVSK+FPV
Sbjct: 122 ENAIFGQPEITIGTIPGLGGTQRWPRYVSKSVAMEICLSGDRLGAQEAKEDGLVSKVFPV 181
Query: 186 ETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREG 245
+ LV EA+ A++IA NS +IV K S+N+A++ +L +G ++EK+LF STFAT+DRREG
Sbjct: 182 QQLVGEAVLLADRIAKNSPLIVKTVKRSLNSAYQTSLNQGLEMEKQLFQSTFATNDRREG 241
Query: 246 MSAFVEKR 253
MSAF EKR
Sbjct: 242 MSAFAEKR 249
^ = sptrembl|Q89QT8|Q89QT8 Enoyl CoA hydratase.//:trembl|AP005946|AP005946_46
gene: "fadB"; product: "enoyl CoA hydratase"; Bradyrhizobium
japonicum USDA 110 DNA, complete genome, section 12/31.
//:gp|AP005946|27351294 enoyl CoA hydratase [Bradyrhizobium
japonicum USDA 110]
Length = 259
0 60 120 180 240
| | | | | |259
sptrembl|Q89QT8|Q89QT | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 734 (263.4 bits), Expect = 2.9e-72, P = 2.9e-72
Identities = 149/255 (58%), Positives = 191/255 (74%)
Query: 1 ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGG 60
+ F++II E +G +VG+I+LNRPK LNAL G+ E+ A++ E D A+G IV+TG
Sbjct: 2 STFEHIIVESQG---AVGIIKLNRPKMLNALSFGVFREIAAAVDDLEGDDAIGCIVVTGS 58
Query: 61 EKAFAAGWDIKEMQNRTFQDCYSGKFLS-HWDHITRIKKPVIAAVNGYALGGGCELAMMC 119
EKAFAAG DIKEMQ + F D +S F + D + R +KP IAAV GYALGGGCELAMMC
Sbjct: 59 EKAFAAGADIKEMQPKGFIDMFSEDFAAIGGDRVARCRKPTIAAVAGYALGGGCELAMMC 118
Query: 120 DIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLV 179
D I A + A+FGQPEI LGTIPG GGTQRLTRA+GKS AM++ LTG + A +A+++GLV
Sbjct: 119 DFIIAADTAKFGQPEITLGTIPGIGGTQRLTRAIGKSKAMDLCLTGRMMDAAEAERSGLV 178
Query: 180 SKIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFAT 239
S+I P + L++E + AEKIA+ S+ VAMAKE+VN AFE TL EG +E+ LF+STFA
Sbjct: 179 SRIVPADKLMDEVMAAAEKIASMSRPAVAMAKEAVNRAFETTLAEGMSVERNLFHSTFAL 238
Query: 240 DDRREGMSAFVEKRK 254
+DR EGM+AF+EKRK
Sbjct: 239 EDRSEGMAAFIEKRK 253
^ = sptremblnew|CAE27252|CAE27252 Enoyl CoA
hydratase.//:tremblnew|BX572598|BX572598_241 product: "enoyl CoA
hydratase"; Rhodopseudomonas palustris CGA009 complete genome;
segment 6/16 //:gp|BX572598|39648730 enoyl CoA hydratase
[Rhodopseudomonas palustris CGA009]
Length = 259
0 60 120 180 240
| | | | | |259
sptremblnew|CAE27252| | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 727 (261.0 bits), Expect = 1.6e-71, P = 1.6e-71
Identities = 148/257 (57%), Positives = 190/257 (73%)
Query: 1 ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGG 60
+ F++II E++ ++VG+I+LNRPK LNAL G+ E+ A+E E D A+G I++TG
Sbjct: 2 STFEFIIVERQ---NAVGIIKLNRPKMLNALSFGVFREIGAAVEDLEADDAIGCILITGS 58
Query: 61 EKAFAAGWDIKEMQNRTFQDCYSGKFLS-HWDHITRIKKPVIAAVNGYALGGGCELAMMC 119
EKAFAAG DIKEMQ + F D ++ F S D + R +KP +AAV GYALGGGCELAMMC
Sbjct: 59 EKAFAAGADIKEMQPKGFIDMFNEDFTSIGGDRLARCRKPTVAAVAGYALGGGCELAMMC 118
Query: 120 DIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLV 179
DII A + A+FGQPEI LGTIPG GGTQRLTRA+GK AM++ LTG + AQ+A+++GLV
Sbjct: 119 DIIIAADTAKFGQPEITLGTIPGIGGTQRLTRAIGKYKAMDLCLTGRMMDAQEAERSGLV 178
Query: 180 SKIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFAT 239
S+I P + L+EEA+ AEKIA S+ AMAK +VN A E TL EG +E+ LF STFA
Sbjct: 179 SRIVPADKLMEEALGAAEKIATMSRPTAAMAKSAVNRALETTLAEGLAVERDLFRSTFAL 238
Query: 240 DDRREGMSAFVEKRKAN 256
+DR EGM+AF+EKRK N
Sbjct: 239 EDRAEGMAAFIEKRKPN 255
^ = sptrembl|Q8XVF9|Q8XVF9 Putative enoyl-CoA hydratase protein (EC
4.2.1.17).//:trembl|AL646072|AL646072_71 gene: "paaF"; "RSc2872";
"RS00230"; product: "PUTATIVE ENOYL-COA HYDRATASE PROTEIN";
Ralstonia solanacearum GMI1000 chromosome, complete sequence;
segment 16/19 //:gp|AL646072|17429895 PUTATIVE ENOYL-COA HYDRATASE
PROTEIN [Ralstonia solanacearum]
Length = 258
0 60 120 180 240
| | | | | |258
sptrembl|Q8XVF9|Q8XVF | __________________________________________________
Local hits (HSPs) | __________________________________________________
Score = 713 (256.0 bits), Expect = 4.9e-70, P = 4.9e-70
Identities = 141/258 (54%), Positives = 194/258 (75%)
Query: 2 NFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGE 61
++Q+I+ E +G+ VGL+ LNRPKALNAL + L++EL AL F+ D +GA+++TG +
Sbjct: 2 SYQHILVETRGR---VGLVTLNRPKALNALNDALMDELGAALLAFDADEGIGAMIITGSD 58
Query: 62 KAFAAGWDIKEMQNRTFQDCYSGKFLS-HWDHITRIKKPVIAAVNGYALGGGCELAMMCD 120
KAFAAG DI M + F Y ++++ +W+ I RI+KPVIAAV GYALGGGCELAMMCD
Sbjct: 59 KAFAAGADIGAMADYDFATVYKNEYITRNWETIRRIRKPVIAAVAGYALGGGCELAMMCD 118
Query: 121 IIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS 180
II A + A+FGQPEI LGT+PGAGGTQRL RAV K+ AM++ LT + A +A++AGLVS
Sbjct: 119 IILAADTARFGQPEIKLGTMPGAGGTQRLPRAVSKAKAMDLCLTARMMGADEAERAGLVS 178
Query: 181 KIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATD 240
++ P + L++EA+ AE IA S M KES+NAA+E TL+EG +LE++LF++TFAT+
Sbjct: 179 RVIPADKLLDEALAAAETIAGFSLPTAMMIKESINAAYETTLSEGVRLERRLFHATFATE 238
Query: 241 DRREGMSAFVEKRKANFK 258
D++EGM AF+ KR+ FK
Sbjct: 239 DQKEGMHAFLGKRQPAFK 256
^ = sptrembl|Q9AHX8|Q9AHX8 FadB1x.//:trembl|AF290950|AF290950_5 gene: "fadB1x";
product: "FadB1x"; Pseudomonas putida FadDx (fadDx), FadB2x
(fadB2x), FadAx (fadAx), FadFx (fadFx), and FadB1x (fadB1x) genes,
complete cds. //:gp|AF290950|13310130 FadB1x [Pseudomonas putida]
Length = 257
0 60 120 180 240
| | | | | |257
sptrembl|Q9AHX8|Q9AHX | __________________________________________________
Local hits (HSPs) | __________________________________________________
Score = 706 (253.6 bits), Expect = 2.7e-69, P = 2.7e-69
Identities = 141/257 (54%), Positives = 190/257 (73%)
Query: 3 FQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEK 62
F+ I+ + GK VGLI LNRP+ALNAL ++ E+NQAL+ E DP++G +VLTG K
Sbjct: 3 FETILLDIHGK---VGLITLNRPQALNALNAQIVGEINQALDQLERDPSIGCVVLTGSAK 59
Query: 63 AFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDII 122
AFAAG DIKEM + + Y S D I +KP+IAAV+G+ALGGGCELAMMCD I
Sbjct: 60 AFAAGADIKEMADLQYPQIYVDDLFSDADRIANRRKPIIAAVSGFALGGGCELAMMCDFI 119
Query: 123 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 182
A + A+FGQPEI LG +PG GGTQRLTRAVGK+ AME+ LTG + A++A++AGLV++I
Sbjct: 120 LAADNAKFGQPEINLGVLPGMGGTQRLTRAVGKAKAMELCLTGRLMGAEEAERAGLVARI 179
Query: 183 FPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDR 242
P LVEEA++ A IA+ S + M KESVN AFE+TL+EG + E+++F++ F+T+D+
Sbjct: 180 VPQAELVEEALKVAATIASKSIPVSMMVKESVNRAFEVTLSEGVRFERRVFHAAFSTEDQ 239
Query: 243 REGMSAFVEKRKANFKD 259
+EGM+AF+ KR+A FK+
Sbjct: 240 KEGMAAFIAKREAQFKN 256
^ = sptrembl|Q9AC55|Q9AC55 Enoyl-CoA hydratase/isomerase family
protein.//:trembl|AE005675|AE005675_6 gene: "CC0006"; product:
"enoyl-CoA hydratase/isomerase family protein"; Caulobacter
crescentus CB15 section 1 of 359 of the complete genome.
//:pironly|F87249|F87249 enoyl-CoA hydratase/isomerase family
protein [imported] - Caulobacter crescentus//:gp|AE005675|13421092
enoyl-CoA hydratase/isomerase family protein [Caulobacter
crescentus CB15]
Length = 262
0 60 120 180 240
| | | | | |262
sptrembl|Q9AC55|Q9AC5 | __________________________________________________
Local hits (HSPs) | __________________________________________________
Score = 704 (252.9 bits), Expect = 4.4e-69, P = 4.4e-69
Identities = 147/259 (56%), Positives = 185/259 (71%)
Query: 1 ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGG 60
A FQ +I E V LI+LNRP+ALNAL L+ EL QAL + D +VG IVLTG
Sbjct: 2 AQFQTLIVEAPESAPGVTLIRLNRPEALNALNTALLGELAQALAAAQADDSVGCIVLTGS 61
Query: 61 EKAFAAGWDIKEMQNRTFQDCYSGKFLSHWDH-ITRIKKPVIAAVNGYALGGGCELAMMC 119
KAFAAG DIKEM ++T+ + F + I + +KP+IAAV GYALGGGCELAMMC
Sbjct: 62 AKAFAAGADIKEMSDKTYAQMFKADFFTAGARAIEQCRKPIIAAVAGYALGGGCELAMMC 121
Query: 120 DIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLV 179
D I A + A+FGQPEI LG PG GGTQRLTR VGKS AM+M+LTG + A++A+++GLV
Sbjct: 122 DFILAADTAKFGQPEINLGVAPGIGGTQRLTRFVGKSKAMDMILTGRMMGAEEAERSGLV 181
Query: 180 SKIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFAT 239
S+IFP ++LV+E + A KIA S + VAM KE V AA+E TLT G LE++LF+S FA
Sbjct: 182 SRIFPADSLVDETLAIAAKIAGQSPLAVAMNKELVEAAYETTLTTGVALERRLFHSLFAF 241
Query: 240 DDRREGMSAFVEKRKANFK 258
+D++EGM+AFVEKRK FK
Sbjct: 242 EDQKEGMTAFVEKRKPLFK 260
^ = sptrembl|Q880J4|Q880J4 Enoyl-CoA hydratase/isomerase family
protein.//:trembl|AE016867|AE016867_98 product: "enoyl-CoA
hydratase/isomerase family protein"; Pseudomonas syringae pv.
tomato str. DC3000 section 12 of 21 of the complete genome.
//:gp|AE016867|28853581 enoyl-CoA hydratase/isomerase family
protein [Pseudomonas syringae pv. tomato str. DC3000]
Length = 257
0 60 120 180 240
| | | | | |257
sptrembl|Q880J4|Q880J | __________________________________________________
Local hits (HSPs) | __________________________________________________
Score = 701 (251.8 bits), Expect = 9.2e-69, P = 9.2e-69
Identities = 141/258 (54%), Positives = 187/258 (72%)
Query: 2 NFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGE 61
+++ I++E G SVGL+ LNRPKALNAL LI+ELN AL+ FE D A+G IVLTG +
Sbjct: 2 DYETILSEVVG---SVGLVTLNRPKALNALNARLIDELNHALDQFEADSAIGCIVLTGTQ 58
Query: 62 KAFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDI 121
KAFAAG DIKEM ++ Y D + +KP++AAV G+ALGGGCELA+MCD
Sbjct: 59 KAFAAGADIKEMVELSYPQIYLDDLFRESDRVAARRKPMVAAVAGFALGGGCELALMCDF 118
Query: 122 IYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSK 181
I A E A+FGQPEI LG +PG GGTQRLTRAVGK+ AMEM LTG I A +A++AGLVS+
Sbjct: 119 ILAAENARFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCLTGRLIDAHEAERAGLVSR 178
Query: 182 IFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDD 241
+ P++ L+ EA+ A IA S + M KESVN AFE++L EG + E+++F++ FA+ D
Sbjct: 179 VLPLDQLLPEALAVAATIAAKSLPVAMMVKESVNRAFEVSLAEGIRFERRVFHAAFASHD 238
Query: 242 RREGMSAFVEKRKANFKD 259
++EGM AF++KR+ +FKD
Sbjct: 239 QKEGMQAFIDKRQPDFKD 256
^ = sptrembl|Q7VU52|Q7VU52 Putative enoyl-CoA
hydratase.//:trembl|BX640421|BX640421_17 product: "putative
enoyl-CoA hydratase"; Bordetella pertussis strain Tohama I,
complete genome; segment 11/12 //:gp|BX640421|33564252 putative
enoyl-CoA hydratase [Bordetella pertussis] [Bordetella pertussis
Tohama I]
Length = 275
0 60 120 180 240
| | | | | |275
sptrembl|Q7VU52|Q7VU5 | __________________________________________________
Local hits (HSPs) | _____________________________________________
Score = 694 (249.4 bits), Expect = 5.1e-68, P = 5.1e-68
Identities = 137/255 (53%), Positives = 189/255 (74%)
Query: 5 YIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAF 64
+++ E +G+ VG + LNRPKALNAL + L+EEL AL + D +GAIV+TG EKAF
Sbjct: 22 FVLVETRGR---VGWLTLNRPKALNALNDQLMEELGAALLALDADDGIGAIVITGSEKAF 78
Query: 65 AAGWDIKEMQNRTFQDCYSGKFLS-HWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIY 123
AAG DI M++ ++ D Y +++ +W+ I R++KPVIAAV GYALGGGCELAMMCDI+
Sbjct: 79 AAGADIGAMKDWSYMDVYRSDYITRNWETIKRVRKPVIAAVAGYALGGGCELAMMCDIVI 138
Query: 124 AGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIF 183
A + A+FGQPEI LG IPGAGGTQRL RAVGK+ AM++ LT + A +A++AGLVS++
Sbjct: 139 AADSARFGQPEIKLGVIPGAGGTQRLPRAVGKAKAMDLALTARMMDAAEAERAGLVSRVV 198
Query: 184 PVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRR 243
P + L +EA++ A IA+ S V MAKE +N A+E+ L +G E+++F+S FAT+D++
Sbjct: 199 PADRLADEALEAATVIASMSLPSVMMAKECINRAYEVPLNDGLLFERRVFHSLFATEDQK 258
Query: 244 EGMSAFVEKRKANFK 258
EGM+AF EKRK +FK
Sbjct: 259 EGMAAFTEKRKPDFK 273
^ = tremblnew|AE017230|AE017230_140 gene: "echA8"; product: "EchA8_1";
Mycobacterium avium subsp. paratuberculosis str. k10, section 4 of
16 of the complete genome.
Length = 263
0 60 120 180 240
| | | | | |263
tremblnew|AE017230|AE | __________________________________________________
Local hits (HSPs) | ________________________________________________
Score = 693 (249.0 bits), Expect = 6.5e-68, P = 6.5e-68
Identities = 136/255 (53%), Positives = 182/255 (71%)
Query: 3 FQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEK 62
F+ I+ E++ + VG+I LNRPKALNAL ++ E+ A F+ DP +GAI++TG K
Sbjct: 9 FETILVEREER---VGIITLNRPKALNALNTQVMNEVTAAATDFDADPGIGAIIITGSAK 65
Query: 63 AFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDII 122
AFAAG DIKEM TF D Y F + W + ++ P IAAV G+ALGGGCELAMMCD++
Sbjct: 66 AFAAGADIKEMAELTFADAYGADFFAPWGKLAAVRTPTIAAVAGHALGGGCELAMMCDVL 125
Query: 123 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 182
A + A+FGQPEI LG +PG GG+QRLTRA+GK+ AM+++LTG I A +A+++GLVS++
Sbjct: 126 IAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRV 185
Query: 183 FPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDR 242
P + L+ EA + A I+ S+ MAKE+VN AFE TL EG E++LF+STFAT D+
Sbjct: 186 VPADDLLTEAKKVATTISQMSRSAARMAKEAVNRAFETTLAEGLLYERRLFHSTFATADQ 245
Query: 243 REGMSAFVEKRKANF 257
EGM+AFVEKR NF
Sbjct: 246 TEGMAAFVEKRAPNF 260
^ = swissnew|O53418|ECH8_MYCTU Probable enoyl-CoA hydratase echA8 (EC
4.2.1.17).//:swiss|O53418|ECH8_MYCTU Probable enoyl-CoA hydratase
echA8 (EC 4.2.1.17).//:trembl|AX023846|AX023846_1 unnamed ORF;
Sequence 17 from Patent WO0021983. //:trembl|AE006991|AE006991_5
gene: "MT1100"; product: "enoyl-CoA hydratase/isomerase family
protein"; Mycobacterium tuberculosis CDC1551, section 77 of 280
of the complete genome. //:trembl|BX248337|BX248337_187 gene:
"echA8"; product: "PROBABLE ENOYL-CoA HYDRATASE ECHA8 (ENOYL
HYDRASE) (UNSATURATED ACYL-CoA HYDRATASE) (CROTONASE)";
Mycobacterium bovis subsp. bovis AF2122/97 complete genome; segment
4/14 //:trembl|BX842575|BX842575_146 gene: "echA8"; product:
"PROBABLE ENOYL-CoA HYDRATASE ECHA8 (ENOYL HYDRASE) (UNSATURATED
ACYL-CoA HYDRATASE) (CROTONASE)"; Mycobacterium tuberculosis H37Rv
complete genome; segment 4/13 //:gp|AX023846|10184191 unnamed
protein product [Mycobacterium tuberculosis]//:gp|BX248337|31617849
PROBABLE ENOYL-CoA HYDRATASE ECHA8 (ENOYL HYDRASE) (UNSATURATED
ACYL-CoA HYDRATASE) (CROTONASE) [Mycobacterium bovis subsp. bovis
AF2122/97]//:gp|AE006991|13880680 enoyl-CoA hydratase/isomerase
family protein [Mycobacterium tuberculosis CDC1551]
Length = 257
0 60 120 180 240
| | | | | |257
swissnew|O53418|ECH8_ | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 666 (239.5 bits), Expect = 4.7e-65, P = 4.7e-65
Identities = 128/255 (50%), Positives = 182/255 (71%)
Query: 3 FQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEK 62
++ I+ E+ + VG+I LNRP+ALNAL + ++ E+ A ++DP +GAI++TG K
Sbjct: 3 YETILVER---DQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAK 59
Query: 63 AFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDII 122
AFAAG DIKEM + TF D ++ F + W + ++ P IAAV GYALGGGCELAMMCD++
Sbjct: 60 AFAAGADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVL 119
Query: 123 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 182
A + A+FGQPEI LG +PG GG+QRLTRA+GK+ AM+++LTG + A +A+++GLVS++
Sbjct: 120 IAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRV 179
Query: 183 FPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDR 242
P + L+ EA A I+ S MAKE+VN AFE +L+EG E++LF+S FAT+D+
Sbjct: 180 VPADDLLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAFATEDQ 239
Query: 243 REGMSAFVEKRKANF 257
EGM+AF+EKR F
Sbjct: 240 SEGMAAFIEKRAPQF 254
^ = swiss|O07137|ECH8_MYCLE Probable enoyl-CoA hydratase echA8 (EC
4.2.1.17).//:trembl|Y13803|MLB1306_5 product: "B1306.05c protein";
Mycobacterium leprae cosmid B1306 DNA
//:trembl|AL583925|MLEPRTN9_136 gene: "ML2402"; product: "putative
enoyl-CoA hydratase/isomerase"; Mycobacterium leprae strain TN
complete genome; segment 9/10 //:gp|Y13803|2198495 B1306.05c
protein [Mycobacterium leprae]//:gp|AL583925|13093985 putative
enoyl-CoA hydratase/isomerase [Mycobacterium leprae]
Length = 257
0 60 120 180 240
| | | | | |257
swiss|O07137|ECH8_MYC | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 658 (236.7 bits), Expect = 3.3e-64, P = 3.3e-64
Identities = 128/241 (53%), Positives = 173/241 (71%)
Query: 17 VGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQNR 76
VG+I LNRP+ALNAL + ++ E+ A + + DP VGAI++TG K FAAG DIKEM +
Sbjct: 14 VGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITGSPKVFAAGADIKEMASL 73
Query: 77 TFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEIL 136
TF D + F S W + ++ P+IAAV GYALGGGCELAMMCD++ A + A+FGQPEI
Sbjct: 74 TFTDAFDADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADTAKFGQPEIK 133
Query: 137 LGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQCA 196
LG +PG GG+QRLTRA+GK+ AM+++LTG I A +A+++GLVS++ + L+ EA A
Sbjct: 134 LGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRVVLADDLLPEAKAVA 193
Query: 197 EKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKAN 256
I+ S+ MAKE+VN +FE TL EG E++LF+STF TDD+ EGM+AF+EKR
Sbjct: 194 TTISQMSRSATRMAKEAVNRSFESTLAEGLLHERRLFHSTFVTDDQSEGMAAFIEKRAPQ 253
Query: 257 F 257
F
Sbjct: 254 F 254
^ = sptrembl|O84978|O84978 PaaA.//:trembl|AF029714|AF029714_4 gene: "paaA";
product: "PaaA"; Pseudomonas putida transcriptional activator
homolog, PaaX (paaX), PaaY (paaY), PaaA (paaA), PaaB (paaB), PaaC
(paaC), PaaE (paaE), PaaF (paaF), PaaG (paaG), PaaH (paaH), PaaI
(paaI), PaaJ (paaJ), PaaK (paaK), PaaL (paaL), PaaM (paaM), PaaN
(paaN), hypothetical two-components response regulator protein,
hypothetical protein, hypothetical protein, and hypothetical
protein genes, complete cds; and unknown gene.
//:gp|AF029714|22711872 PaaA [Pseudomonas putida]
Length = 257
0 60 120 180 240
| | | | | |257
sptrembl|O84978|O8497 | __________________________________________________
Local hits (HSPs) | _______________________________________________
Score = 609 (219.4 bits), Expect = 5.2e-59, P = 5.2e-59
Identities = 130/244 (53%), Positives = 160/244 (65%)
Query: 15 SSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQ 74
+ V LI L RP+ALNALC L+ EL AL+ D +G +VLTG KAFAAG DI+EM
Sbjct: 12 NGVQLITLQRPEALNALCTELLAELATALDAAARDDQIGVVVLTGSRKAFAAGADIREMA 71
Query: 75 NRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPE 134
R + ++HW I KP+IAAVNGYALGGGCEL M DI+ AG A+FGQPE
Sbjct: 72 ERDLVGILNDPRVAHWQRIAAFAKPLIAAVNGYALGGGCELVMCADIVIAGSDARFGQPE 131
Query: 135 ILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQ 194
I LG IPGAGGTQRL RAVGK LAM+MVLTG+ I+A+ A+QAGLVS+I E VE A+Q
Sbjct: 132 INLGIIPGAGGTQRLLRAVGKPLAMQMVLTGEAITARHAQQAGLVSEITQPEFTVERAMQ 191
Query: 195 CAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRK 254
A IA + + V +AKE++ A + L G + E+ F T DR EG+ AF EKR
Sbjct: 192 IARNIAAKAPLAVRLAKEALLKAGDTDLASGLRFERHAFTLLAGTADRDEGIQAFQEKRP 251
Query: 255 ANFK 258
A F+
Sbjct: 252 ARFQ 255
^ = sptremblnew|CAE45102|CAE45102 Putative enoyl-CoA hydratase
I.//:trembl|AJ579894|PSP579894_4 gene: "paaA2"; product: "putative
enoyl-CoA hydratase I"; Pseudomonas sp. Y2 paa2 gene cluster for
phenylacetate degradation //:gp|AJ579894|33945692 putative
enoyl-CoA hydratase I [Pseudomonas sp. Y2]
Length = 256
0 60 120 180 240
| | | | | |256
sptremblnew|CAE45102| | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 602 (217.0 bits), Expect = 2.8e-58, P = 2.8e-58
Identities = 130/240 (54%), Positives = 161/240 (67%)
Query: 19 LIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQNRTF 78
LI L+RP ALNAL L+ EL L+ +DP V A+V+TG KAFAAG D+ EM R
Sbjct: 15 LITLHRPHALNALTTELLGELADELQRCADDPDVRAVVITGSRKAFAAGADLNEMAERDL 74
Query: 79 QDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLG 138
+W IT+ KP+IAAVNGYALGGGCELAM DI+ AGE AQFGQPEI LG
Sbjct: 75 VGMLDDPRQGYWQRITQFPKPLIAAVNGYALGGGCELAMHADILIAGEDAQFGQPEINLG 134
Query: 139 TIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQCAEK 198
+PGAGGTQRL RAVGKSLAM+MVL G+ ISA+ A AGLVS+I E VE A+Q A++
Sbjct: 135 IMPGAGGTQRLLRAVGKSLAMQMVLAGESISARRALDAGLVSEITQPELTVERALQIAQR 194
Query: 199 IANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKANFK 258
IA + + V +AKE++ A ++ L G + E+ F T DR EG+ AF EKR+A+FK
Sbjct: 195 IAAKAPLAVRLAKEALLKAQDLDLASGLRFERHAFTLLAGTADRDEGIRAFQEKRQADFK 254
^ = sptrembl|Q88HR9|Q88HR9 Enoyl-CoA
hydratase/isomerase.//:trembl|AE016786|AE016786_88 product:
"enoyl-CoA hydratase/isomerase"; Pseudomonas putida KT2440 section
13 of 21 of the complete genome. //:gp|AE016786|24984922 enoyl-CoA
hydratase/isomerase [Pseudomonas putida KT2440]
Length = 257
0 60 120 180 240
| | | | | |257
sptrembl|Q88HR9|Q88HR | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 591 (213.1 bits), Expect = 4.2e-57, P = 4.2e-57
Identities = 130/242 (53%), Positives = 159/242 (65%)
Query: 17 VGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQNR 76
V LI L RP+ALNALC L+ EL AL+ D V A V+TG KAFAAG DI+EM +R
Sbjct: 14 VQLITLQRPEALNALCTELLAELAAALQAAGNDEHVRATVITGSAKAFAAGADIREMADR 73
Query: 77 TFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEIL 136
+ ++HW I KP+IAAVNGYALGGGCELAM DI+ A A+FGQPEI
Sbjct: 74 DLVGILNDPRVAHWQSIAAFAKPLIAAVNGYALGGGCELAMCADIVIASTDARFGQPEIN 133
Query: 137 LGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQCA 196
LG IPGAGGTQRL RAVGK LAM+MVLTG+ I+A A+QAGLVS+I E VE A+Q A
Sbjct: 134 LGIIPGAGGTQRLLRAVGKPLAMQMVLTGEAITALRAQQAGLVSEITQPELTVERAMQVA 193
Query: 197 EKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKAN 256
IA + + V +AKE++ A + L G + E+ F T DR EG+ AF EKR+A
Sbjct: 194 RSIAAKAPLAVRLAKEALLKAGDTDLASGLRFERHAFTLLAGTADRDEGIRAFQEKRQAR 253
Query: 257 FK 258
F+
Sbjct: 254 FQ 255
^ = sptrembl|Q9Z9V2|Q9Z9V2 Enoyl CoA hydratase (Enoyl-CoA hydratase subunit I in
the phenylacetic acid catabolic
pathway).//:trembl|AB011837|AB011837_7 gene: "yngF"; product:
"enoyl CoA hydratase"; Bacillus halodurans C-125 genomic DNA,
clone ALBAC005. //:trembl|AP001507|AP001507_201 gene: "BH0201";
product: "enoyl-CoA hydratase"; Bacillus halodurans genomic DNA,
section 1/14. //:pironly|A83675|A83675 enoyl-CoA hydratase chain I
phaA [imported] - Bacillus halodurans (strain
C-125)//:gp|AB011837|4512366 enoyl CoA hydratase [Bacillus
halodurans]//:gp|AP001507|10172813 enoyl-CoA hydratase [Bacillus
halodurans]
Length = 257
0 60 120 180 240
| | | | | |257
sptrembl|Q9Z9V2|Q9Z9V | __________________________________________________
Local hits (HSPs) | _______________________________________________
Score = 583 (210.3 bits), Expect = 2.9e-56, P = 2.9e-56
Identities = 118/243 (48%), Positives = 163/243 (67%)
Query: 16 SVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQN 75
+VG+IQLNRPK NAL +++E+ + E ++ D + I++ G E+AFAAG DI+EM+
Sbjct: 13 AVGVIQLNRPKVANALNRQMVQEIVEQAERYDRDQEIRVILVKGSERAFAAGADIREMEG 72
Query: 76 RTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEI 135
T + WD TRI KP++AAV+G+ALGGG ELA+ CD+I A A+FG PE+
Sbjct: 73 ETPIRFELADPFADWDRFTRIHKPMVAAVSGFALGGGFELALHCDLIVAATNARFGFPEV 132
Query: 136 LLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQC 195
LG +PGAGGTQ LTRAVG+ A+E + TG ISAQ+A Q G+++KI E + EEA +
Sbjct: 133 SLGVLPGAGGTQLLTRAVGRKKALEWIWTGKHISAQEALQIGIINKIVEPELVEEEAFRL 192
Query: 196 AEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKA 255
A I + I V + KE+V+ A ++TL +G LE+K FY FAT D++EGM AF+EKR
Sbjct: 193 ARTIVKQAPIAVRLIKEAVHQAEDVTLADGMMLERKNFYLAFATKDQKEGMKAFLEKRPP 252
Query: 256 NFK 258
FK
Sbjct: 253 IFK 255
^ = sptrembl|Q845K2|Q845K2 Putative enoyl CoA-hydratase
I.//:trembl|AJ000330|PSSTYCATA_12 gene: "paaA"; product: "putative
enoyl-CoA hydratase I"; Pseudomonas sp. Y2 DNA for styrene
catabolism genes //:gp|AJ000330|28974559 putative enoyl-CoA
hydratase I [Pseudomonas sp. Y2]
Length = 257
0 60 120 180 240
| | | | | |257
sptrembl|Q845K2|Q845K | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 573 (206.8 bits), Expect = 3.4e-55, P = 3.4e-55
Identities = 124/241 (51%), Positives = 155/241 (64%)
Query: 17 VGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQNR 76
V LI L RP+ALNAL L++EL L E+D A+VLTG KAFAAG DIKEM R
Sbjct: 14 VRLITLQRPEALNALNTQLLDELAAELALAEQDAETRAVVLTGSRKAFAAGADIKEMAER 73
Query: 77 TFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEIL 136
++HW I KP+IAAVNG+ LGGGCELAM DI+ AGE A+FGQPEI
Sbjct: 74 DLVGILEDPRVAHWQRIAAFSKPLIAAVNGFCLGGGCELAMHADILIAGEDARFGQPEIN 133
Query: 137 LGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQCA 196
LG +PGAGGTQRL RAVGKSLAM+MVL+G I A+ A++AGLVS++ E +E A+ A
Sbjct: 134 LGIMPGAGGTQRLLRAVGKSLAMQMVLSGQAIDARHAQRAGLVSEVTLPELTIERALAIA 193
Query: 197 EKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKAN 256
IA + + V +AKE++ A + L G + E+ F T DR EG+ AF EKR+
Sbjct: 194 RVIAQKAPLAVRLAKEALLKAEDTDLASGLRFERHAFTVLAGTADRAEGIRAFQEKRRPE 253
Query: 257 F 257
F
Sbjct: 254 F 254
^ = swiss|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF (EC
4.2.1.17).//:trembl|AE000236|ECAE236_7 gene: "ydbS"; product:
"putative enzyme"; Escherichia coli K12 MG1655 section 126 of 400
of the complete genome. //:gp|AE000236|1787659 putative enzyme
[Escherichia coli K12]
Length = 255
0 60 120 180 240
| | | | | |255
swiss|P76082|PAAF_ECO | __________________________________________________
Local hits (HSPs) | ________________________________________________
Score = 571 (206.1 bits), Expect = 5.5e-55, P = 5.5e-55
Identities = 117/246 (47%), Positives = 162/246 (65%)
Query: 13 KNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKE 72
+ V L+ LNRP A NAL N L+ +L LE D ++ V+TG + FAAG D+ E
Sbjct: 8 RQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFAAGADLNE 67
Query: 73 MQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQ 132
M + + W + KP+IAAVNGYALG GCELA++CD++ AGE A+FG
Sbjct: 68 MAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGL 127
Query: 133 PEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEA 192
PEI LG +PGAGGTQRL R+VGKSLA +MVL+G+ I+AQ A+QAGLVS +FP + +E A
Sbjct: 128 PEITLGIMPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVFPSDLTLEYA 187
Query: 193 IQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEK 252
+Q A K+A +S + + AK+++ + E+ L G E++LF AT+DR EG+SAF++K
Sbjct: 188 LQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEGISAFLQK 247
Query: 253 RKANFK 258
R +FK
Sbjct: 248 RTPDFK 253
^ = sptrembl|O07453|O07453 Cyclohex-1-ene-1-carboxyl-CoA
hydratase.//:sptremblnew|CAE26094|CAE26094
Cyclohex-1-ene-1-carboxyl-CoA hydratase (EC
4.2.1.17).//:tremblnew|BX572595|BX572595_19 gene: "badK"; product:
"cyclohex-1-ene-1-carboxyl-CoA hydratase"; Rhodopseudomonas
palustris CGA009 complete genome; segment 3/16
//:trembl|U75363|RPU75363_1 gene: "badk"; product:
"cyclohex-1-ene-1-carboxyl-CoA hydratase"; Rhodopseudomonas
palustris cyclohex-1-ene-1-carboxyl-CoA hydratase (badk),
cyclohex-1-ene-1-carboxylate CoA ligase (aliA),
cyclohexanecarboxyl-CoA dehydrogenase (badJ),
2-ketocyclohexanecarboxyl-CoA hydrolase (badI), putative
2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (badH), putative
regulatory protein (badR), putative NADPH:quinone oxidoreductase
(badC), and benzoyl-CoA reductase subunits (badD), (badE), (badF)
and (badG) genes, complete cds. //:pironly|T51760|T51760
cyclohex-1-ene-1-carboxyl-CoA hydratase (EC 4.2.1.-) [imported] -
Rhodopseudomon//:gp|U75363|3046310 cyclohex-1-ene-1-carboxyl-CoA
hydratase [Rhodopseudomonas palustris]//:gp|BX572595|39647574
cyclohex-1-ene-1-carboxyl-CoA hydratase [Rhodopseudomonas palustris
CGA009]
Length = 258
0 60 120 180 240
| | | | | |258
sptrembl|O07453|O0745 | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 570 (205.7 bits), Expect = 7.0e-55, P = 7.0e-55
Identities = 120/253 (47%), Positives = 167/253 (66%)
Query: 6 IITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFA 65
I+TE +G+ VG+I LNRP LNAL + L++ L AL F+ D +GAIV+ G +AFA
Sbjct: 6 ILTETQGR---VGIITLNRPDVLNALNDALMDALGGALLAFDADDGIGAIVIAGNTRAFA 62
Query: 66 AGWDIKEMQNRTFQDCYSGKFLS-HWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYA 124
AG DI M ++ D Y F++ +W+ I +I+KPV+AAV G A GGGCELA+ CDI+ A
Sbjct: 63 AGADIASMAAWSYSDVYGSNFITRNWETIRQIRKPVLAAVAGLAYGGGCELALACDIVIA 122
Query: 125 GEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFP 184
G A+F PEI LG +PGAGGTQRL RA+GK+ AM+M L+ ++A++A + GLVS++
Sbjct: 123 GRSAKFALPEIKLGLLPGAGGTQRLPRAIGKAKAMDMCLSARPLNAEEADRYGLVSRVVD 182
Query: 185 VETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRRE 244
+ L +E + A IA S + KES+N AFE TL EG E++ ++ FA+ D RE
Sbjct: 183 DDRLRDETVALATTIAAFSAPALMALKESLNRAFESTLAEGILFERRELHARFASADARE 242
Query: 245 GMSAFVEKRKANF 257
G+ AF+EKR F
Sbjct: 243 GIQAFLEKRAPCF 255
^ = sptrembl|Q96XV1|Q96XV1 Putative enoyl-CoA
hydratase.//:trembl|AP000990|AP000990_14 gene: "ST2416"; product:
"258aa long hypothetical enoyl-CoA hydratase"; Sulfolobus tokodaii
genomic DNA, complete sequence, section:10/10.
//:gp|AP000990|15623539 258aa long hypothetical enoyl-CoA hydratase
[Sulfolobus tokodaii]
Length = 258
0 60 120 180 240
| | | | | |258
sptrembl|Q96XV1|Q96XV | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 565 (203.9 bits), Expect = 2.4e-54, P = 2.4e-54
Identities = 115/243 (47%), Positives = 155/243 (63%)
Query: 16 SVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQN 75
++ +++LNRP LNAL ++ + E + V +++TG +AF AG D+ EM N
Sbjct: 15 NIAIVRLNRPDKLNALNMEMVWDFVNVFNELENNKNVKVVIITGNGRAFCAGADVNEMAN 74
Query: 76 RTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEI 135
+D W+ + +KPVIAA+NG +GGG ELAM CDII A E A GQPEI
Sbjct: 75 MKVEDVVRVGHAPMWERLRTFRKPVIAALNGLTVGGGLELAMACDIIIAAESAMLGQPEI 134
Query: 136 LLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQC 195
LG IPGAGGTQRLTR VGK MEMVLTG ISA +A + GLV K+ P E L++EAI+
Sbjct: 135 NLGIIPGAGGTQRLTRTVGKYKGMEMVLTGRLISAWEAYRRGLVIKVVPDEALLDEAIRL 194
Query: 196 AEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKA 255
A++IA S V + KE+VN A + TL +G +E++ FY ++D +EGM AF+EKRK
Sbjct: 195 AKEIATKSLFAVELGKEAVNKALDTTLQQGLDIERRNFYVALISEDGKEGMKAFIEKRKP 254
Query: 256 NFK 258
N+K
Sbjct: 255 NWK 257
^ = sptrembl|Q8RC92|Q8RC92 Enoyl-CoA hydratase/carnithine
racemase.//:trembl|AE013024|AE013024_11 gene: "CaiD"; product:
"Enoyl-CoA hydratase/carnithine racemase"; Thermoanaerobacter
tengcongensis strain MB4T, section 51 of 244 of the complete
genome. //:gp|AE013024|20515532 Enoyl-CoA hydratase/carnithine
racemase [Thermoanaerobacter tengcongensis]
Length = 260
0 60 120 180 240
| | | | | |260
sptrembl|Q8RC92|Q8RC9 | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 563 (203.2 bits), Expect = 3.9e-54, P = 3.9e-54
Identities = 119/245 (48%), Positives = 158/245 (64%)
Query: 17 VGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAFAAGWDIKEMQN 75
+ I +NRPKALNAL +EEL LE + D V IV+TG GEKAF AG DI EM++
Sbjct: 14 IATITINRPKALNALNTETLEELENVLEVLQNDDGVKVIVITGAGEKAFVAGADISEMKD 73
Query: 76 RTFQDCYSGKFLSH--WDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQP 133
+ + L + I +KKPVIAAVNGYALGGGCELA+ CDI A A+FGQP
Sbjct: 74 MSVFEAKKFAELGQKVFRKIELVKKPVIAAVNGYALGGGCELALACDIRIASRNAKFGQP 133
Query: 134 EILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAI 193
E+ LG IPG GGTQRL R VG S A E++ TGD I A++A + GL+SK+ + L+EEA
Sbjct: 134 EVGLGIIPGFGGTQRLPRIVGVSKAKELIYTGDMIDAEEALRIGLISKVVEQDKLLEEAY 193
Query: 194 QCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKR 253
A+KI + + V++AKE++N + E+ + G + E F F T D++EGM+AF+EKR
Sbjct: 194 GIAKKIMSKGLVAVSLAKEAINKSLEVDIDSGMEYEANAFAMCFGTQDQKEGMAAFLEKR 253
Query: 254 KANFK 258
F+
Sbjct: 254 APKFE 258
^ = sptrembl|Q891F2|Q891F2 Putative crotonase.//:trembl|AE015944|AE015944_130
gene: "CTC02427"; product: "putative crotonase"; Clostridium
tetani E88, section 9 of 10 of the complete genome.
//:gp|AE015944|28204455 putative crotonase [Clostridium tetani E88]
Length = 260
0 60 120 180 240
| | | | | |260
sptrembl|Q891F2|Q891F | __________________________________________________
Local hits (HSPs) | __________________________________________________
Score = 560 (202.2 bits), Expect = 8.0e-54, P = 8.0e-54
Identities = 118/261 (45%), Positives = 175/261 (67%)
Query: 2 NFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGE 61
+F+ +I EK GK + ++ +NRP ALNAL + L++EL+ A++ ED V A+VLTG
Sbjct: 2 DFKNLILEKDGK---IAVLTINRPDALNALNSELLKELDYAIDCLAEDDEVLAVVLTGAG 58
Query: 62 KAFAAGWDIKEMQNRTFQDCYSGKFLSH--WDHITRIKKPVIAAVNGYALGGGCELAMMC 119
KAF AG DI EM++ T + L + + + ++KPVIAAVNG+ALGGGCE++M C
Sbjct: 59 KAFVAGADIGEMKDLTVSEGRKFGVLGNRVFRKLETLEKPVIAAVNGFALGGGCEISMAC 118
Query: 120 DIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLV 179
DI A EKA+FGQPE+ LG PG GGTQRL R VG ++A E++ T D I A++A + GL+
Sbjct: 119 DIRIASEKAKFGQPEVGLGITPGFGGTQRLPRLVGPAVAKELIYTADIIGAEEALRIGLI 178
Query: 180 SKIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFAT 239
+KI + L+++A + A KIANN+ I + + K ++N + + G E ++F F+T
Sbjct: 179 NKIVEKDELLDKAKEMANKIANNAPIAIKLCKSAINRGVQCDIDTGIAFEAEVFGECFST 238
Query: 240 DDRREGMSAFVEKRKAN-FKD 259
+D++EGM+AF+EKRK FK+
Sbjct: 239 EDQKEGMTAFLEKRKDKCFKN 259
^ = sptrembl|P97087|P97087 Crotonase (EC 4.2.1.17).//:trembl|Z92974|TTBCSOPRN_1
gene: "crt"; product: "crotonase"; T.thermosaccharolyticum BCS
operon DNA //:gp|Z92974|1903327 crotonase [Thermoanaerobacterium
thermosaccharolyticum]
Length = 259
0 60 120 180 240
| | | | | |259
sptrembl|P97087|P9708 | __________________________________________________
Local hits (HSPs) | ________________________________________________
Score = 558 (201.5 bits), Expect = 1.3e-53, P = 1.3e-53
Identities = 117/250 (46%), Positives = 163/250 (65%)
Query: 13 KNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAFAAGWDIK 71
K+ + LI +NRPKALNAL ++EL+ L+ E D + +++TG GEK F AG DI
Sbjct: 10 KDDGIALIIINRPKALNALNYETLKELDSVLDIVENDKEIKVLIITGSGEKTFVAGADIA 69
Query: 72 EMQNRTFQDCYSGKFLSHWDHITR----IKKPVIAAVNGYALGGGCELAMMCDIIYAGEK 127
EM N T + + KF + + R + KPVIAAVNG+ALGGGCEL+M CDI A +
Sbjct: 70 EMSNMTPLE--AKKFSLYGQKVFRKIEMLSKPVIAAVNGFALGGGCELSMACDIRIASKN 127
Query: 128 AQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVET 187
A+FGQPE+ LG IPG GTQRL R +G S A E++ TGD I++ +A + GL+SK+ +
Sbjct: 128 AKFGQPEVGLGIIPGFSGTQRLPRLIGTSKAKELIFTGDMINSDEAYKIGLISKVVELSD 187
Query: 188 LVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMS 247
L+EEA + A+K+ + S+I +++AKE++N E L GN +E + F F TDD++EGM
Sbjct: 188 LIEEAKKLAKKMMSKSQIAISLAKEAINKGMETDLDTGNTIEAEKFSLCFTTDDQKEGMI 247
Query: 248 AFVEKRKANF 257
AF EKR F
Sbjct: 248 AFSEKRAPKF 257
^ = sptrembl|Q97VK0|Q97VK0 Enoyl CoA hydratase (paaF-7) (EC
4.2.1.17).//:trembl|AE006858|AE006858_6 gene: "paaF-7"; product:
"Enoyl CoA hydratase (paaF-7)"; Sulfolobus solfataricus section
217 of 272 of the complete genome. //:pironly|A90436|A90436 enoyl
CoA hydratase (paaF-7) [imported] - Sulfolobus
solfataricus//:gp|AE006858|13815932 Enoyl CoA hydratase (paaF-7)
[Sulfolobus solfataricus]
Length = 266
0 60 120 180 240
| | | | | |266
sptrembl|Q97VK0|Q97VK | __________________________________________________
Local hits (HSPs) | _____________________________________________
Score = 555 (200.4 bits), Expect = 2.7e-53, P = 2.7e-53
Identities = 110/244 (45%), Positives = 161/244 (65%)
Query: 16 SVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQN 75
++G+I+LNRP LNA+ +++EL L + D + +++TG KAF+AG D+KEM
Sbjct: 19 NIGIIKLNRPDKLNAINFQMVDELVDVLNKLDNDDKIKVVIITGNGKAFSAGADVKEMLE 78
Query: 76 RTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEI 135
++ + W+ + KKPVIAA+NG GGG ELAM CDII A E A+ GQPEI
Sbjct: 79 TPLEEIMKKGHMPLWEKLRTFKKPVIAALNGITAGGGLELAMACDIIIASESAKLGQPEI 138
Query: 136 LLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQC 195
LG +PGAGGTQRLTR +GK AME+VLTG I +++A++ GLV+K+ P +L++EAI+
Sbjct: 139 NLGIMPGAGGTQRLTRVLGKYKAMELVLTGKLIDSKEAERYGLVNKVVPDNSLIDEAIRL 198
Query: 196 AEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKA 255
A +IA I + +AKE+V A++ L +G E++ FY T + +EGM AF+EKRK
Sbjct: 199 AREIAEKPIISIILAKEAVARAWDTLLQQGLDFERRNFYLALNTKEAKEGMRAFLEKRKP 258
Query: 256 NFKD 259
+++
Sbjct: 259 RWEN 262
^ = sptrembl|Q8XI23|Q8XI23 3-hydroxybutryl-CoA
dehydratase.//:trembl|AP003193|AP003193_206 gene: "crt"; product:
"3-hydroxybutryl-CoA dehydratase"; Clostridium perfringens str. 13
DNA, complete genome, section 9/10. //:gp|AP003193|18145966
3-hydroxybutryl-CoA dehydratase [Clostridium perfringens str. 13]
Length = 260
0 60 120 180 240
| | | | | |260
sptrembl|Q8XI23|Q8XI2 | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 553 (199.7 bits), Expect = 4.4e-53, P = 4.4e-53
Identities = 114/257 (44%), Positives = 172/257 (66%)
Query: 6 IITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAF 64
+I EK+G ++G++ +NRPKALNAL + +++L+ A++ E+ + ++LTG G+KAF
Sbjct: 6 VIFEKEG---NIGVLTINRPKALNALNSETLKDLDTAIDHIEKQDDIYVVILTGAGDKAF 62
Query: 65 AAGWDIKEMQNRTFQDCYSGKFLSH--WDHITRIKKPVIAAVNGYALGGGCELAMMCDII 122
AG DI EM++ ++ L + + + + KPVIAA+NG+ALGGGCE++M CDI
Sbjct: 63 VAGADIAEMKDLNEEEGKEFGLLGNKVFRRLENLDKPVIAAINGFALGGGCEISMACDIR 122
Query: 123 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 182
A KA+F QPE+ LG PG GGTQRL R VG A E++ TGD I A +A + GLV+K+
Sbjct: 123 IATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKELIYTGDMIKADEALRIGLVNKV 182
Query: 183 FPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDR 242
E L+EEA+ A+KI+NN+ I V + K+++N ++ + +E + F FAT+D+
Sbjct: 183 VEPENLMEEAMSLAKKISNNAPIAVKLCKDAINRGIQVDIDSAVVIEAEDFGKCFATEDQ 242
Query: 243 REGMSAFVEKRKANFKD 259
EGMSAFVE+R+ NFK+
Sbjct: 243 TEGMSAFVERREKNFKN 259
^ = sptrembl|Q98LI4|Q98LI4 Enoyl-CoA hydratase.//:trembl|AP002996|AP002996_115
gene: "mll1009"; product: "enoyl-CoA hydratase"; Mesorhizobium
loti DNA, complete genome, section 3/21. //:gp|AP002996|14021868
enoyl-CoA hydratase [Mesorhizobium loti]
Length = 258
0 60 120 180 240
| | | | | |258
sptrembl|Q98LI4|Q98LI | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 546 (197.3 bits), Expect = 2.4e-52, P = 2.4e-52
Identities = 116/242 (47%), Positives = 151/242 (62%)
Query: 17 VGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQNR 76
+ L+ LNRP LNAL L+ EL+ L ++ D VG +VLTG +AFAAG DI +M R
Sbjct: 15 IRLLTLNRPDKLNALSKALLAELSHLLSGYDADTEVGCVVLTGAGRAFAAGADISDMLER 74
Query: 77 TFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEIL 136
+ L+ W I KP+IAAVNGYALGGG ELA++CDI+ A + AQF PEI
Sbjct: 75 GVASYADPERLACWRAIEGFTKPIIAAVNGYALGGGLELALLCDIVIASQAAQFATPEIK 134
Query: 137 LGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQCA 196
+G PG GGTQRL R VGKS AM+MVLTGD + A A++ GLVS++ + L+ A++ A
Sbjct: 135 IGAFPGDGGTQRLPRLVGKSFAMQMVLTGDMVDATLAERKGLVSEVVEADRLLPRALEIA 194
Query: 197 EKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKAN 256
IA S I AK++V AAFE L G ++E +L F +DR EG+ AF EKR
Sbjct: 195 AAIAAKSVAITPYAKKAVLAAFETELQSGLEIEHRLTVEAFGKEDRIEGLRAFAEKRAPA 254
Query: 257 FK 258
F+
Sbjct: 255 FR 256
^ = sptrembl|Q970T6|Q970T6 Putative 3-hydroxybutyryl-CoA
dehydratase.//:trembl|AP000986|AP000986_202 gene: "ST1516";
product: "269aa long hypothetical 3-hydroxybutyryl-CoA
dehydratase"; Sulfolobus tokodaii genomic DNA, complete sequence,
section:6/10. //:gp|AP000986|15622596 269aa long hypothetical
3-hydroxybutyryl-CoA dehydratase [Sulfolobus tokodaii]
Length = 269
0 60 120 180 240
| | | | | |269
sptrembl|Q970T6|Q970T | __________________________________________________
Local hits (HSPs) | _____________________________________________
Score = 542 (195.9 bits), Expect = 6.5e-52, P = 6.5e-52
Identities = 119/250 (47%), Positives = 160/250 (64%)
Query: 13 KNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDI-- 70
K + +G I LNRP LNA+ +I+EL Q ++ D + I++TG KAF+AG DI
Sbjct: 20 KETPIGWIYLNRPDRLNAINQQMIKELRQGIDEMVYDSDIKVIIITGNGKAFSAGADISR 79
Query: 71 -KEMQNRT-FQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKA 128
KE+ T +Q SG+ L D+I I KP IA VNGYALGGG ELAM CDI A E+A
Sbjct: 80 FKELNGYTAWQFAKSGRELM--DYIENISKPTIAMVNGYALGGGLELAMACDIRIAAEEA 137
Query: 129 QFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETL 188
Q G PEI LG PG GGTQRL R +GK A+E++LTGDRISA++A++ GLV+K+ P+ L
Sbjct: 138 QLGLPEINLGIYPGFGGTQRLVRLIGKGKALELMLTGDRISAKEAEKIGLVNKVVPLSNL 197
Query: 189 VEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSA 248
+E A K+A I +A+ K VN ++ + G +E + F+T+D +EG+SA
Sbjct: 198 EQETRNFALKLAEKPPISIALIKLLVNQGIDLPILAGLNMESLGWGVVFSTEDEKEGVSA 257
Query: 249 FVEKRKANFK 258
F+EKRKA FK
Sbjct: 258 FLEKRKAQFK 267
^ = sptrembl|Q8BU95|Q8BU95 Enoyl coenzyme A
hydratase.//:trembl|AK086762|AK086762_1 Mus musculus 15 days embryo
head cDNA, RIKEN full-length enriched library, clone:D930049N01
product:enoyl Coenzyme A hydratase, short chain, 1, mitochondrial,
full insert sequence. //:gp|AK086762|26352205 unnamed protein
product [Mus musculus]
Length = 153
0 30 60 90 120 150
| | | | | |153
sptrembl|Q8BU95|Q8BU9 | __________________________________________________
Local hits (HSPs) | ___________________________________
Score = 539 (194.8 bits), Expect = 1.3e-51, P = 1.3e-51
Identities = 103/109 (94%), Positives = 107/109 (98%)
Query: 1 ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGG 60
ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFE+DPAVGAIVLTGG
Sbjct: 31 ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEQDPAVGAIVLTGG 90
Query: 61 EKAFAAGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYAL 109
+KAFAAG DIKEMQNRTFQDCYS KFLSHWDHITR+KKPVIAAVNGYA+
Sbjct: 91 DKAFAAGADIKEMQNRTFQDCYSSKFLSHWDHITRVKKPVIAAVNGYAV 139
^ = sptremblnew|AAR34753|AAR34753 3-hydroxybutyryl-CoA
dehydratase.//:tremblnew|AE017211|AE017211_248 product:
"3-hydroxybutyryl-CoA dehydratase"; Geobacter sulfurreducens PCA,
section 5 of 13 of the complete genome. //:gp|AE017211|39983359
3-hydroxybutyryl-CoA dehydratase [Geobacter sulfurreducens PCA]
Length = 260
0 60 120 180 240
| | | | | |260
sptremblnew|AAR34753| | __________________________________________________
Local hits (HSPs) | _______________________________________________
Score = 538 (194.4 bits), Expect = 1.7e-51, P = 1.7e-51
Identities = 119/249 (47%), Positives = 156/249 (62%)
Query: 14 NSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAFAAGWDIKE 72
+ + I +NRP A+NA+ ++EL +A+ P V A +LTG G KAF AG DI
Sbjct: 11 SEGIAAITINRPSAMNAMTPATLDELAEAVRRVNGAPEVRAAILTGAGTKAFMAGADIAA 70
Query: 73 MQNRTFQDCYSGKFLSH--WDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQF 130
M++ T +H + I R K IAAVNGYALGGGCELAM CDI A E A+F
Sbjct: 71 MRDMTPAQARDLARQAHQIYADIERSPKTFIAAVNGYALGGGCELAMACDIRLASENAKF 130
Query: 131 GQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVE 190
GQPEI +G IPG GGTQRL R VGK A+EM+LTG+ I A++A + GLV+++ E L E
Sbjct: 131 GQPEINIGIIPGFGGTQRLPRLVGKGRALEMILTGEMIDAREAHRIGLVNRVVTQEELPE 190
Query: 191 EAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFV 250
EA + A IA + V + KE+VN M LT+ E +LF +F+T D++EGMSAF+
Sbjct: 191 EARRLARAIAAKGMVAVGLCKEAVNNGLNMELTKACAYEAELFAHSFSTADQKEGMSAFL 250
Query: 251 EKRKANFKD 259
EKR A F+D
Sbjct: 251 EKRPAVFRD 259
^ = sptrembl|Q9I393|Q9I393 Probable enoyl-CoA
hydratase/isomerase.//:trembl|AE004590|AE004590_9 product:
"probable enoyl-CoA hydratase/isomerase"; Pseudomonas aeruginosa
PAO1, section 151 of 529 of the complete genome.
//:pironly|H83442|H83442 probable enoyl-CoA hydratase/isomerase
PA1629 [imported] - Pseudomonas aeruginos//:gp|AE004590|9947596
probable enoyl-CoA hydratase/isomerase [Pseudomonas aeruginosa
PAO1]
Length = 261
0 60 120 180 240
| | | | | |261
sptrembl|Q9I393|Q9I39 | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 537 (194.1 bits), Expect = 2.2e-51, P = 2.2e-51
Identities = 112/244 (45%), Positives = 154/244 (63%)
Query: 15 SSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQ 74
+ + L++LNRP+A NAL + + + L +T DPA+ IVLTG + FAAG D++++
Sbjct: 16 ADIALLRLNRPQARNALNDEVRQRLASHFQTLGADPAIRVIVLTGDSRCFAAGADLRDLS 75
Query: 75 NRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPE 134
T Y +W+ I R KPVIAAVNG+ALGGGCELAM CD+I AGE AQF QPE
Sbjct: 76 TSTAIGLYGRHGERYWEAIARCPKPVIAAVNGFALGGGCELAMHCDLIVAGESAQFAQPE 135
Query: 135 ILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQ 194
I +G +PGAGGTQRL RAVGK A+ M+ TG + A A GLVS++ E+ + A++
Sbjct: 136 IKVGVMPGAGGTQRLVRAVGKFQALRMLFTGCLVKAPQALAMGLVSEVVADESTLARALE 195
Query: 195 CAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRK 254
A +IA + +A KE V A ++ L LE+K F F + D++EGM AF+EKR
Sbjct: 196 LAMEIARLPPLALAQIKEVVLAGADLPLDSALALERKAFQLLFDSQDQKEGMHAFLEKRP 255
Query: 255 ANFK 258
N++
Sbjct: 256 PNYQ 259
^ = sptrembl|O29299|O29299 Enoyl-CoA hydratase
(FAD-3).//:trembl|AE001038|AE001038_14 gene: "AF0963"; product:
"enoyl-CoA hydratase (fad-3)"; Archaeoglobus fulgidus DSM 4304
section 69 of 172 of the complete genome. //:pironly|C69370|C69370
probable enoyl-CoA hydratase (EC 4.2.1.17) fad-3 - Archaeoglobus
fulgidus//:gp|AE001038|2649635 enoyl-CoA hydratase (fad-3)
[Archaeoglobus fulgidus DSM 4304]
Length = 259
0 60 120 180 240
| | | | | |259
sptrembl|O29299|O2929 | __________________________________________________
Local hits (HSPs) | ______________________________________________
Score = 531 (192.0 bits), Expect = 9.5e-51, P = 9.5e-51
Identities = 116/244 (47%), Positives = 158/244 (64%)
Query: 20 IQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQNRTFQ 79
++ NRP+ALNA+ ++ L + ++ + V IVLTG KAF AG DIK +
Sbjct: 15 VKFNRPEALNAINKDFVKGLREVVDYARNNKTVRVIVLTGEGKAFCAGADIKMFSESSHF 74
Query: 80 DCYS-----GKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPE 134
S GK L + + + PVIAA+NG+ALGGGCE+AM CDII A E+A FGQPE
Sbjct: 75 VARSTIEELGKVLEEMEDL---EVPVIAAINGFALGGGCEIAMACDIIIASERASFGQPE 131
Query: 135 ILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQ 194
I LG IPGAGGTQRL R VG AME+ LTG+RISA++A + GLV+K+ + L++EA +
Sbjct: 132 INLGIIPGAGGTQRLARIVGWKKAMELCLTGERISAEEAYRLGLVNKVVEHDKLMDEAKK 191
Query: 195 CAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRK 254
AE I + S V + K++VN F+M L +G E+ LF +F++ D EG+ AFVEKRK
Sbjct: 192 MAEVIKSKSPYAVMLVKQAVNRGFKMGLRDGIMYERDLFALSFSSPDAEEGIKAFVEKRK 251
Query: 255 ANFK 258
A+F+
Sbjct: 252 ADFR 255
^ = tremblnew|BX682953|EBN1C002_3 gene: "orf2"; product: "Enoyl-CoA hydratase";
Azoarcus sp. like strain EbN1, total orf 35 predicted gene,
involved in anaerobic degradation of toluene
Length = 256
0 60 120 180 240
| | | | | |256
tremblnew|BX682953|EB | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 531 (192.0 bits), Expect = 9.5e-51, P = 9.5e-51
Identities = 119/253 (47%), Positives = 157/253 (62%)
Query: 6 IITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFA 65
++ E+ G + L++LNRP A NAL + ++L F +DP V IVLTGG+K FA
Sbjct: 4 VLIERPGPG--IVLLRLNRPDARNALNQEVRQQLATHFTAFGQDPDVRCIVLTGGDKFFA 61
Query: 66 AGWDIKEMQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAG 125
AG DI+ M + D W I KPVIAAVNGYA GGGCELAM DII AG
Sbjct: 62 AGADIRAMADAGAIDMMLRHTHRLWQAIASCPKPVIAAVNGYAWGGGCELAMHADIIVAG 121
Query: 126 EKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPV 185
E A F QPE+ +G +PGAGGTQRLTRAVGK AM+MVLTG ++ +DA + GL S++
Sbjct: 122 ESASFCQPEVKVGIMPGAGGTQRLTRAVGKFKAMKMVLTGQPVNGRDALEMGLASEVVAD 181
Query: 186 ETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREG 245
+ A++ A +IA + +A KE + A + +L LE+K F FA+ D++EG
Sbjct: 182 ADVQAHAVELAAQIAALPPLAIAQIKEVLIAGQDASLETALMLERKAFQLLFASRDQKEG 241
Query: 246 MSAFVEKRKANFK 258
M AF+EKRKANF+
Sbjct: 242 MQAFLEKRKANFE 254
^ = tremblnew|BX842651|BX842651_35 Bdellovibrio bacteriovorus complete genome,
strain HD100; segment 6/11
Length = 265
0 60 120 180 240
| | | | | |265
tremblnew|BX842651|BX | __________________________________________________
Local hits (HSPs) | __________________________________________________
Score = 528 (190.9 bits), Expect = 2.0e-50, P = 2.0e-50
Identities = 116/263 (44%), Positives = 168/263 (63%)
Query: 1 ANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEE-DPA-VGAIVLT 58
+N++ I+ E+K V ++ +NRP++LNAL + ++ E+ +AL E D + A+++T
Sbjct: 3 SNYKTILLEQK--THGVWVLTINRPESLNALNSTVLNEMGEALRQIGEMDYSDARALIIT 60
Query: 59 G-GEKAFAAGWDIKEMQNRTFQDC--YSGKFLSHWDHITRIKKPVIAAVNGYALGGGCEL 115
G GEKAF AG DIKE+ + + ++ + S + +T +K PVIAAVNG+ALGGGCEL
Sbjct: 61 GAGEKAFVAGADIKEIHDLDEEKALVFAQRGQSIFHELTLLKIPVIAAVNGFALGGGCEL 120
Query: 116 AMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQ 175
A+ CD IYA E A+FG PE+ LG IPG GGT R+ RAVG A E+ TG I+A +A
Sbjct: 121 ALGCDFIYAAENAKFGLPEVSLGLIPGFGGTVRMARAVGSRRARELTYTGGMITAAEALS 180
Query: 176 AGLVSKIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYS 235
AGLV+K+ P L+ ++ E I + I V AK S+N A++M + E K E ++F
Sbjct: 181 AGLVNKVVPQAELMNTVMKTVEAILAKAPIAVGSAKFSINQAWDMDVEEAQKNEARIFAE 240
Query: 236 TFATDDRREGMSAFVEKRKANFK 258
F ++D +EG AF+EKRKA FK
Sbjct: 241 LFTSEDVKEGTGAFIEKRKAEFK 263
^ = sptrembl|O29814|O29814 Enoyl-CoA hydratase
(FAD-1).//:trembl|AE001074|AE001074_2 gene: "AF0435"; product:
"enoyl-CoA hydratase (fad-1)"; Archaeoglobus fulgidus DSM 4304
section 33 of 172 of the complete genome. //:pironly|C69304|C69304
probable enoyl-CoA hydratase (EC 4.2.1.17) fad-1 - Archaeoglobus
fulgidus//:gp|AE001074|2650190 enoyl-CoA hydratase (fad-1)
[Archaeoglobus fulgidus DSM 4304]
Length = 256
0 60 120 180 240
| | | | | |256
sptrembl|O29814|O2981 | __________________________________________________
Local hits (HSPs) | ________________________________________________
Score = 523 (189.2 bits), Expect = 6.7e-50, P = 6.7e-50
Identities = 119/248 (47%), Positives = 156/248 (62%)
Query: 14 NSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEM 73
+ + + LNRP+ LNAL EL + +E EE V +++TG KAFAAG DI E+
Sbjct: 10 DGEIAVATLNRPEKLNALDTKTRMELAEVIEGIEEVARV--LIITGSGKAFAAGADINEL 67
Query: 74 QNRTFQDCYSGKFLSHWDHITRIKK---PVIAAVNGYALGGGCELAMMCDIIYAGEKAQF 130
R + L D +RI++ PVIAAVNGY LGGGCELAM CDI A EKA+F
Sbjct: 68 LQRDAIKAFEATKLGT-DLFSRIEELEIPVIAAVNGYTLGGGCELAMACDIRIASEKAKF 126
Query: 131 GQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVE 190
GQPEI L IPGAGGTQRL R VG +A ++VLTG+ I AQ A + GLV ++ E L+E
Sbjct: 127 GQPEINLAIIPGAGGTQRLPRLVGLGMAKKLVLTGEIIDAQTALRIGLVEEVVEHERLME 186
Query: 191 EAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFV 250
A + A KI S + V +AK+++NA+ M L EG + E LF F+++D +EGM AF+
Sbjct: 187 RAKEVAAKIIEKSPLAVKVAKKALNASINMPLKEGLRYEASLFALLFSSEDAKEGMRAFL 246
Query: 251 EKRKANFK 258
EKRK F+
Sbjct: 247 EKRKPEFR 254
^ = sptrembl|Q9F7E7|Q9F7E7 Enoyl CoA dehydratase (Enoyl-CoA hydratase-like
protein).//:trembl|AB026669|AB026669_5 gene: "orf14"; product:
"enoyl-CoA hydratase-like protein"; Acinetobacter sp. NCIMB9871
cyclohexanol metabolic gene cluster (orf18, orf17, orf16, orf15,
orf14, orf13, orf12, chnB), partial and complete cds.
//:trembl|AF282240|AF282240_2 gene: "fadB"; product: "enoyl CoA
dehydratase"; Acinetobacter sp. SE19 ChnZ (chnZ), pilin inverting
protein (chnY), ChnX (chnX) genes, complete cds and cyclohexanol
oxidation gene cluster, complete sequence. //:gp|AB026669|14587417
enoyl-CoA hydratase-like protein [Acinetobacter sp.
NCIMB9871]//:gp|AF282240|9965283 enoyl CoA dehydratase
[Acinetobacter sp. SE19]//:gpnew|AB026669|14587417 enoyl-CoA
hydratase-like protein [Acinetobacter sp. NCIMB9871]
Length = 258
0 60 120 180 240
| | | | | |258
sptrembl|Q9F7E7|Q9F7E | __________________________________________________
Local hits (HSPs) | _______________________________________________
Score = 523 (189.2 bits), Expect = 6.7e-50, P = 6.7e-50
Identities = 113/246 (45%), Positives = 151/246 (61%)
Query: 13 KNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKE 72
+++ V +++L+RP NAL L ++L E V AIV+TGGEK FAAG DI++
Sbjct: 11 QSNGVAIVELHRPDTRNALNLELRQQLAAMFEQLAASDTVRAIVITGGEKVFAAGADIRD 70
Query: 73 MQNRTFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQ 132
D Y +W I KP++AAVNGYALGGGCELAM DII AG+ AQFGQ
Sbjct: 71 FTTAKTVDMYLRHTEQYWRAIIDCPKPIVAAVNGYALGGGCELAMHADIIIAGKSAQFGQ 130
Query: 133 PEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEA 192
PE+ LG +PGAGGTQRL RAVGK AM++VLTG SA++A + GLVS++ + + +A
Sbjct: 131 PEVKLGLMPGAGGTQRLLRAVGKFKAMQIVLTGKIFSAEEADKMGLVSEVVEDDQTLAKA 190
Query: 193 IQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEK 252
++ A +IA S I V KE M L LE+K F F T D++EG++AF EK
Sbjct: 191 VEIATQIAQLSPIAVEQIKEVTTLGANMPLDGALALERKAFQILFDTQDQKEGVNAFFEK 250
Query: 253 RKANFK 258
R ++
Sbjct: 251 RSPQYQ 256
^ = sptrembl|Q8XP70|Q8XP70 Crotonase.//:trembl|AP003185|AP003185_95 gene:
"CPE0095"; product: "crotonase"; Clostridium perfringens str. 13
DNA, complete genome, section 1/10. //:gp|AP003185|18143752
crotonase [Clostridium perfringens str. 13]
Length = 258
0 60 120 180 240
| | | | | |258
sptrembl|Q8XP70|Q8XP7 | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 523 (189.2 bits), Expect = 6.7e-50, P = 6.7e-50
Identities = 112/251 (44%), Positives = 159/251 (63%)
Query: 14 NSSVGLIQ--LNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAFAAGWDI 70
N S G+ + +NRPKALNAL N I EL + + + + +++TG GEKAF AG DI
Sbjct: 7 NESNGIAEVIINRPKALNALNNQTITELGEVINEISKRKDIKTVIITGAGEKAFVAGADI 66
Query: 71 KEMQNRTFQDCYSGKFLSH--WDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKA 128
EM++ + L+ + I + + VIAAVNGYALGGGCEL+M CDI A +KA
Sbjct: 67 VEMKDLNSMEARDFSRLAQKVFSDIENMPQIVIAAVNGYALGGGCELSMACDIRLASKKA 126
Query: 129 QFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETL 188
+FGQPE+ LG +PG GTQRL R VGK +A E++ + D I A++A + GL +K++ E L
Sbjct: 127 KFGQPEVNLGILPGFAGTQRLPRLVGKGIAKELIFSTDMIDAEEAHRIGLANKVYEPEEL 186
Query: 189 VEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSA 248
+++A + A KI + S + V +AK ++N M E LF F+T+D+ EGM+A
Sbjct: 187 MDKARELANKIMSKSPVGVRLAKAAINNGLNMDTESAYNYEADLFALCFSTEDQLEGMNA 246
Query: 249 FVEKRKANFKD 259
FV+KRKA+FKD
Sbjct: 247 FVDKRKADFKD 257
^ = sptrembl|Q937T3|Q937T3 DcaE.//:trembl|L05770|ACPCAOP_4 gene: "dcaE";
product: "DcaE"; Acinetobacter sp. ADP1 dca-pca-qui-pob-hca
supraoperonic gene cluster, complete sequence.
//:gp|L05770|15812041 DcaE [Acinetobacter sp. ADP1]
Length = 261
0 60 120 180 240
| | | | | |261
sptrembl|Q937T3|Q937T | __________________________________________________
Local hits (HSPs) | _____________________________________________
Score = 521 (188.5 bits), Expect = 1.1e-49, P = 1.1e-49
Identities = 110/241 (45%), Positives = 153/241 (63%)
Query: 17 VGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQNR 76
+ ++++NRP + NAL + ++L QA + + AIVLTGGE FAAG D+KEM
Sbjct: 18 IAIVKINRPASKNALNTEVRKQLAQAFTELSFNDQINAIVLTGGEDVFAAGADLKEMATA 77
Query: 77 TFQDCYSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEIL 136
+ D +W+ I + KPVIAAVNGYALGGGCELAM DII AG+ A FGQPEI
Sbjct: 78 SSTDMLLRHTERYWNAIAQCPKPVIAAVNGYALGGGCELAMHTDIIIAGKSATFGQPEIK 137
Query: 137 LGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQCA 196
+G +PGAGGTQRL RAVGK AM M++TG + A++A GLVS++ + AI+ A
Sbjct: 138 VGLMPGAGGTQRLFRAVGKFHAMRMIMTGVMVPAEEAYLIGLVSQVTEDSQTIPTAIKMA 197
Query: 197 EKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKAN 256
+ +A I + KE + ++ L G LE+K F F+T+D+ EG++AF+EKRK +
Sbjct: 198 QSLAKMPPIALQQIKEVALMSEDVPLNAGLTLERKSFQLLFSTEDKNEGINAFIEKRKPS 257
Query: 257 F 257
+
Sbjct: 258 Y 258
^ = sptrembl|Q898H3|Q898H3 3-hydroxybutyryl-CoA dehydratase (EC
4.2.1.55).//:trembl|AE015937|AE015937_150 gene: "CTC00479";
product: "3-hydroxybutyryl-coA dehydratase"; Clostridium tetani
E88, section 2 of 10 of the complete genome.
//:gp|AE015937|28202663 3-hydroxybutyryl-coA dehydratase
[Clostridium tetani E88]
Length = 258
0 60 120 180 240
| | | | | |258
sptrembl|Q898H3|Q898H | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 520 (188.1 bits), Expect = 1.4e-49, P = 1.4e-49
Identities = 113/253 (44%), Positives = 158/253 (62%)
Query: 13 KNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAFAAGWDIK 71
K + + I +NRPK+LNAL + + EL A++ E V +++TG GEKAF AG DI
Sbjct: 8 KENGIVEITINRPKSLNALNSETLNELGAAIKDISESDDVKVVIITGAGEKAFVAGADIT 67
Query: 72 EMQNRTFQDC-----YSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGE 126
EMQN + + K S +H+ +I VIAAVNGYALGGGCEL M CDI A +
Sbjct: 68 EMQNLNAKQGRALARLAQKVFSDIEHMPQI---VIAAVNGYALGGGCELCMACDIRLASK 124
Query: 127 KAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVE 186
A+FGQPE+ LG PG GTQRL R VGK A E++ T D I A +A + LV+K++ +
Sbjct: 125 NAKFGQPEVNLGITPGFAGTQRLPRLVGKGTAKELIFTTDMIDANEAYRIQLVNKVYEAD 184
Query: 187 TLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGM 246
L+++A + A+KI + + V++AK ++N M K E +F FAT+D++EGM
Sbjct: 185 ELIDKARELAKKIMSKAPYAVSLAKAAINDGMNMDTESAYKYEADIFGLCFATEDQKEGM 244
Query: 247 SAFVEKRKANFKD 259
AF+EKRK+NFK+
Sbjct: 245 KAFLEKRKSNFKN 257
^ = sptrembl|Q8RMI7|Q8RMI7 Crotonase (EC 4.2.1.17).//:trembl|AF494018|AF494018_1
gene: "crt"; product: "crotonase"; Clostridium beijerinckii strain
NRRL B593 crotonase (crt), butyryl-CoA dehydrogenase (bcd),
electron-transferring flavoprotein beta-subunit (etfB),
electron-transferring flavoprotein alpha-subunit (etfA), and
3-hydroxybutyryl-CoA dehydrogenase (hbd) genes, complete cds; and
probable 8-oxoguanine DNA glycosylase (ogg1) gene, partial cds.
//:gp|AF494018|20162438 crotonase [Clostridium beijerinckii]
Length = 261
0 60 120 180 240
| | | | | |261
sptrembl|Q8RMI7|Q8RMI | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 513 (185.6 bits), Expect = 7.7e-49, P = 7.7e-49
Identities = 113/258 (43%), Positives = 168/258 (65%)
Query: 6 IITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAF 64
+I EK+G + ++ +NRPKALNAL + +++LN L+ E D V A+++TG GEK+F
Sbjct: 6 VILEKEGH---LAIVTINRPKALNALNSETLKDLNVVLDDLEADNNVYAVIVTGAGEKSF 62
Query: 65 AAGWDIKEMQNRTFQDCYSGKFLSH--WDHITRIKKPVIAAVNGYALGGGCELAMMCDII 122
AG DI EM++ + L + + + ++ KPVIAA++G+ALGGGCELAM CDI
Sbjct: 63 VAGADISEMKDLNEEQGKEFGILGNNVFRRLEKLDKPVIAAISGFALGGGCELAMSCDIR 122
Query: 123 YAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKI 182
A KA+FGQPE LG PG GGTQRL R VG A E++ T D I+A++A + GLV+K+
Sbjct: 123 IASVKAKFGQPEAGLGITPGFGGTQRLARIVGPGKAKELIYTCDLINAEEAYRIGLVNKV 182
Query: 183 FPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDR 242
+E L+EEA A KIA N+ VA K++++ ++ + +E + F FAT+D+
Sbjct: 183 VELEKLMEEAKAMANKIAANAPKAVAYCKDAIDRGMQVDIDAAILIEAEDFGKCFATEDQ 242
Query: 243 REGMSAFVEKR-KANFKD 259
EGM+AF+E+R + NF++
Sbjct: 243 TEGMTAFLERRAEKNFQN 260
^ = swiss|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55)
(Crotonase).//:trembl|AE007768|AE007768_12 gene: "CAC2712";
product: "Crotonase (3-hydroxybutyryl-COA dehydratase)";
Clostridium acetobutylicum ATCC824 section 256 of 356 of the
complete genome. //:trembl|U17110|CA17110_1 gene: "crt"; product:
"crotonase"; Clostridium acetobutylicum crotonase (crt), putative
butyryl-CoA dehydrogenase (BCD), putative a-subunit of
electron-transfer flavoprotein (etfA), putative b-subunit of
electron-transfer flavoprotein (etfB), and 3-hydroxybutyryl-CoA
dehydrogenase (hbd) genes, complete cds. //:gp|AE007768|15025745
Crotonase (3-hydroxybutyryl-COA dehydratase) [Clostridium
acetobutylicum]//:gp|U17110|1055218 crotonase [Clostridium
acetobutylicum]
Length = 261
0 60 120 180 240
| | | | | |261
swiss|P52046|CRT_CLOA | __________________________________________________
Local hits (HSPs) | _________________________________________________
Score = 511 (184.9 bits), Expect = 1.3e-48, P = 1.3e-48
Identities = 109/259 (42%), Positives = 164/259 (63%)
Query: 6 IITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTG-GEKAF 64
+I EK+GK V ++ +NRPKALNAL + ++E++ + E D V A++LTG GEK+F
Sbjct: 6 VILEKEGK---VAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSF 62
Query: 65 AAGWDIKEMQN-RTFQDCYSGKFLSH--WDHITRIKKPVIAAVNGYALGGGCELAMMCDI 121
AG DI EM+ T + G L + + + ++KPVIAAVNG+ALGGGCE+AM CDI
Sbjct: 63 VAGADISEMKEMNTIEGRKFG-ILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDI 121
Query: 122 IYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSK 181
A A+FGQPE+ LG PG GGTQRL+R VG +A +++ T I A +A + GLV+K
Sbjct: 122 RIASSNARFGQPEVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNK 181
Query: 182 IFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDD 241
+ L+ A + A KI +N+ + V ++K+++N + + E + F F+T+D
Sbjct: 182 VVEPSELMNTAKEIANKIVSNAPVAVKLSKQAINRGMQCDIDTALAFESEAFGECFSTED 241
Query: 242 RREGMSAFVEKRKAN-FKD 259
+++ M+AF+EKRK FK+
Sbjct: 242 QKDAMTAFIEKRKIEGFKN 260
^ = sptrembl|Q9YBW6|Q9YBW6 659AA long hypothetical 3-hydroxybutyryl-CoA
dehydratase.//:trembl|AP000061|AP000061_347 gene: "APE1484";
product: "659aa long hypothetical 3-hydroxybutyryl-CoA
dehydratase"; Aeropyrum pernix genomic DNA, section 4/7.
//:pironly|D72628|D72628 probable 3-hydroxybutyryl-CoA dehydratase
APE1484 - Aeropyrum pernix (strain K1)//:gp|AP000061|5105168 659aa
long hypothetical 3-hydroxybutyryl-CoA dehydratase [Aeropyrum
pernix]
Length = 659
0 140 280 420 560
| | | | | |659
sptrembl|Q9YBW6|Q9YBW | __________________________________________________
Local hits (HSPs) | __________________
Score = 498 (180.4 bits), Expect = 3.0e-47, P = 3.0e-47
Identities = 114/244 (46%), Positives = 149/244 (61%)
Query: 17 VGLIQLNRPKALNALCNGLIEELNQALETFEEDPAVGAIVLTGGEKAFAAGWDIKEMQNR 76
+ I LNRP LNA+ +I EL+QAL+ EE V A++LTG +AF+AG D+
Sbjct: 414 IAWIVLNRPDKLNAISPKMIMELSQALDELEERSDVRAVILTGAGRAFSAGADVTAFAQV 473
Query: 77 TFQDC--YSGKFLSHWDHITRIKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPE 134
T D +S KF I KPVI A+ GYALGGG ELAM DI A E A GQPE
Sbjct: 474 TPIDILRFSRKFQELTLKIQFYTKPVIVAIKGYALGGGLELAMSGDIRIASEDAMLGQPE 533
Query: 135 ILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQ 194
I LG IPGAGGTQRL R G + A E+++TGD I A DA++ G+V+++ P E L +EA
Sbjct: 534 INLGFIPGAGGTQRLARLAGPARAKELIMTGDMIPASDAEKMGIVNRVVPPELLEQEASS 593
Query: 195 CAEKIANNSKIIVAMAKESVNAAFEMTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRK 254
A K+A I +A AK +++ E + G +LE LF F+T+D EG++AF+EKRK
Sbjct: 594 LALKLAEKPPIALAAAKYAIDFGLESNIWAGLQLEASLFSVLFSTEDVIEGVTAFLEKRK 653
Query: 255 ANFK 258
FK
Sbjct: 654 PKFK 657
WARNING: HSPs involving 751 database sequences were not reported due to the
limiting value of parameter B = 50.
Parameters:
matrix=BLOSUM62
E=10
V=50
B=50
hspmax=10
filter=seg
echofilter
stats
qtype
qres
ctxfactor=1.00
Query ----- As Used ----- ----- Computed ----
Frame MatID Matrix name Lambda K H Lambda K H
+0 0 BLOSUM62 0.317 0.134 0.379 same same same
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
Query
Frame MatID Length Eff.Length E S W T X E2 S2
+0 0 260 260 10. 80 3 11 22 0.24 33
32 0.24 36
Statistics:
Query Expected Observed HSPs HSPs
Frame MatID High Score High Score Reportable Reported
+0 0 71 (32.5 bits) 1312 (600.4 bits) 878 50
Query Neighborhd Word Excluded Failed Successful Overlaps
Frame MatID Words Hits Hits Extensions Extensions Excluded
+0 0 5111 136357320 25971335 110119981 266004 275
Database: /dove2/data/db/nrdb95
Title: nrdb-95%
Release date: Rel. 17.Feb.2004
Posted date: 10:06:02 AM MET Feb 17, 2004
Last modified: unknown
First created: unknown
Format: BLAST-1.4
# of letters in database: 286,239,830
# of sequences in database: 882,049
# of database sequences satisfying E: 801
No. of states in DFA: 561 (110 KB)
Total size of DFA: 222 KB (256 KB)
Time to generate neighborhood: 0.01u 0.01s 0.02t Elapsed: 00:00:00
No. of threads or processors used: 4
Search cpu time: 219.43u 5.01s 224.44t Elapsed: 00:01:54
Total cpu time: 219.54u 5.11s 224.65t Elapsed: 00:01:55
Start: Tue Feb 17 16:36:22 2004 End: Tue Feb 17 16:38:17 2004
WARNINGS ISSUED: 2
Y.P. Yuan Your comments/suggestions are highly appreciated! Thanks