FUGUE v2.s.05 (JUL 2002)
Search sequence(s) against fold library using environment-specific
substitution tables and structure-dependent gap penalties.
Fold library and substitution tables are based on the HOMSTRAD database.
http://www-cryst.bioc.cam.ac.uk/~homstrad/
FUGUE server is available at:
http://www-cryst.bioc.cam.ac.uk/~fugue/
http://www-cryst.bioc.cam.ac.uk/~fugue/prfsearch.html
Citation:
J. Shi, T. L. Blundell and K. Mizuguchi (2001), J Mol Biol, 310(1), 243-57
Size of fold library: 4242
Probe sequence ID : target_1
Probe sequence len : 91
Probe divergence : 0.733
Recommended cutoff : ZSCORE >= 6.0 (CERTAIN 99% confidence)
Other cutoff : ZSCORE >= 4.0 (LIKELY 95% confidence)
Other cutoff : ZSCORE >= 3.5 (MARGINAL 90% confidence)
Other cutoff : ZSCORE >= 2.0 (GUESS 50% confidence)
Other cutoff : ZSCORE < 2.0 (UNCERTAIN)
PLEN : Profile length
RAWS : Raw alignment score
RVN : (Raw score)-(Raw score for NULL model)
ZSCORE : Z-score normalized by sequence divergence
ZORI : Original Z-score (before normalization)
AL : Alignment algorithm used for Zscore/Alignment calculation
0 -- Global, 2 -- GloLocSeq (No sequence termini gap penalty)
3 -- GloLocPrf (No profile termini gap penalty)
The sequence(s) you submitted is HERE (in original format).
The sequence(s) actually used by FUGUE is HERE (in PIR format).
Download all the results in compressed format HERE. new!
View Ranking (Click on a profile hit will bring you to the corresponding HOMSTRAD family)
Profile Hit PLEN RAWS RVN ZSCORE ZORI AL
hsd1ail 70 -6 59 2.60 4.20 00 GUESS Alignment hs1bxpb 13 -65 8 1.94 3.25 02 UNCERTAIN Alignment hsd1bf2_3 475 -786 45 1.92 4.69 03 UNCERTAIN Alignment hsd1cd1a2 179 -175 21 1.88 3.48 03 UNCERTAIN Alignment hs1n2fa 142 -104 6 1.85 4.69 03 UNCERTAIN Alignment hs1hgva 46 19 17 1.75 3.07 22 UNCERTAIN Alignment Ribosomal_S30AE 125 -48 31 1.67 4.35 00 UNCERTAIN Alignment hs1gbrb 15 -68 15 1.66 2.98 02 UNCERTAIN Alignment hs1hlva 131 -85 51 1.64 4.51 00 UNCERTAIN Alignment hsd1uok_2 479 -791 25 1.63 4.32 03 UNCERTAIN Alignment
View Alignments (What do they mean -- aa, ma, mh, hh? See note at the bottom of the page.)
Hint: check 'ma' first if your query is a single sequence, otherwise start with 'aa'. Profile Hit HTML POSTSCRIPT TEXT(PIR FORMAT) Confidence View Model Z-score hsd1ail aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.60 hs1bxpb aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.94 hsd1bf2_3 aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.92 hsd1cd1a2 aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.88 hs1n2fa aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.85 hs1hgva aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.75 Ribosomal_S30AE aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.67 hs1gbrb aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.66 hs1hlva aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.64 hsd1uok_2 aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.63Keys
aa: query sequences (including PSI-BLAST homologues) aligned against all the representative structures from a HOMSTRAD family ma: master sequence aligned against all the representative structures from a HOMSTRAD family mh: master sequence aligned against a single structure of highest sequence identity from a HOMSTRAD family hh: single sequence/structure pair with highest sequence identity in 'aa' Note: If your query is a single sequence, master sequence is equivelent to your query and all the other sequences (if any) are collected by PSI-BLAST. If your query is a sequence alignment, master sequence is set to the first sequence in the alignment.JOY Keys are described here
If you have any further questions, please write to Jiye Shi and Kenji Mizuguchi at fugue@cryst.bioc.cam.ac.uk