ERa-ERb Ligand Binding Domains (EST, RAL, OHT, & GEN)

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  1. Compare ERa-EST to:
B. ERa-RAL
C. ERa-OHT
D. ERb-GEN
E. ERa-EST (mutant)
F. ERa-EST (tetramer)
  2. ADD Chains A-F to current display:
A. ERa-EST
B. ERa-RAL
C. ERa-OHT
D. ERb-GEN
E. ERa-EST (mutant)
F. ERa-EST (tetramer)
Aligned Structures of ERa-EST with Several ER-Antagonist (or SERM) Complexes.
Chain A: The ERa-EST complex is Ribbons, colored green. Helices are numbered at their N-termini. Residues from the end of helix 11 to the C-terminus are Backbone, colored dark green. Helix 12 is thicker Backbone ("H12A"); EST is Spacefill, colored CPK.
Chains B-F: The ER complexes are Backbone, colored gray. Residues from the end of helix 11 to the C-terminus are color-coded. Helix 12 is thick Backbone (RAL, OHT, and GEN in the original structures are not shown.)
The numbered "Highlight" buttons above display Helix 12 positioning comparisons to ERa-EST either as 1. pairwise or as 2. additive comparisons. The following paragraphs briefly describe the displays.

1. ERa-EST compared separately to the other ER LDB's
ER-Ligand   Color   [PDB ID] Sequences of Colored Segments (Helix 12) 
    A. ERa-EST   Green   [1ere.pdb]  231 KNVVPLYDLLLEMLDAHR 248
    B. ERa-RAL   Orange   [1err.pdb]  235 ....PLYDLLLEMLDAH 247
    C. ERa-OHT   Red   [3ert.pdb]  238 KNVVPLYDLLLEMLDAHRLHA 258
    D. ERb-GEN   Yellow   [1qkm.pdb]  228 KNVVPVYDLLLEMLNAHVL 246
    E. ERa-EST (mutant)   Blue   [1qkt.pdb]  228 KNVVPLYDLLLEMLDAHRLHA 248
    F. ERa-EST (tetramer)   Cyan   [1a52.pdb]  235 KNVVPLYDLLLEML 248
Note: The table shows the residue numbers and aligned sequences of the color-coded segments of each LBD. Spaces in these entries correspond to missing residues in the structure models. In addition, "Helix 12" is defined slightly differently in several models. The underlined segments are those reported in the structure papers. The residue numbering here for ERa-EST is 300 less than standard ERa numbering, e.g. Helix 12 starts at Asp 538 (D238, here) and ends at His 547 (H247).

2. ADD Chains A-F to current display allows more than two models to be viewed. The display and color coding are the same as in the pairwise comparison displays. 

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The estrogen receptor structure papers are cited on the pages linked below.

Structure alignments were done at the Combinatorial Extension Server.

Additional ER structures:
A. Estrogen Receptora   ERa complexes with bound:
    Estradiol (EST), an agonist.
    Raloxifene (RAL), an antagonist.
    Diethylstilbestrol (DES), an agonist & the NR Box II peptide.
    Tamoxifen (OHT), an antagonist.
    (Estradiol (EST), in an unusual tetrameric structure.)
B. Estrogen Receptorb   ERb complexes with bound:
    Genistein (GEN), a partial agonist.
    Raloxifene (RAL), an antagonist.
C. Structural Alignments   Pairwise superimposed comparisons:
    ERa (EST) and ERb (GEN), ERa vs. ERb in agonist complexes.
    ERa (RAL) and ERb (RAL), ERa vs. ERb in antagonist complexes.
    ERa: DES and OHT, ERa: agonist vs. antagonist complex.
    ERa: EST, Wild type ER vs. a triple mutant (Helix 12 in the antagonist conformation).
    ERa: EST, The Brzozowski, et al. (1997) vs. Tanenbaum, et al. (1998) models.
    "Helix 12 Gallery": ERa-EST vs. All five ER-antagonist (SERM) models.
D. DNA-Binding Domain:
    ER-DBD Complex   Base pair & backbone contacts.

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6.15.01