FLAGdb/FST

http://genoplante-info.infobiogen.fr

Samson, F.¹, Brunaud, V.¹, Balzergue, S.¹, Dubreucq, B.², Lepiniec, L.², Pelletier, G.³, Caboche, M.¹, Lecharny, A.¹

¹URGV, INRA, FRE CNRS, 2, rue Gaston Crчieux, F-91000 Evry, France.
²Laboratoire de Biologie des Semences, INRA, F-78026, Versailles, France.
³Station de Gшюique et Amцioration des Plantes, INRA, F-78026, Versailles, France.

Contact lecharny@ibp.u-psud.fr

Database Description

A large collection of T-DNA insertion transform ants of Arabidopsis thaliana has been generated, at Institute of Agronomic Research, Versailles, France. The molecular characterisation of the insertion sites is currently done by sequencing genome regions flanking the inserted T-DNA (FST) (Balzergue et al., 2001). The almost complete sequence of the nuclear genome of A. thaliana (AGI, 2000) provides the framework for organising FSTs in a genome oriented database, FLAGdb/FST (http://genoplante-info.infobiogen.fr). The main scope of FLAGdb/FST is to help biologists to find the FSTs that interrupt the genes they are interested in. FSTs are anchored to the genome sequences of A. thaliana and positions of both predicted genes and FSTs are shown graphically on sequences. Requests are made using BLAST programs to locate the genomic position of a query sequence. The response delivered by FLAGdb/FST is a graphical representation of the putative FSTs and of predicted genes in a 20 kb long region.

Acknowledgements

We thank the staffs of GENOPLANTE-INFO and INFOBIOGEN for their help and cooperation in maintaining FLAGdb/FST on their site. This work was supported by the French Gшoplante Program.

REFERENCES

Balzergue S., Dubreucq B., Chauvin S., Le-Clainche I., Le Boulaire F., deRose R., Samson F., Biaudet V., Lecharny A., Cruaud C., Weissenbach J., Caboche M. and Lepiniec L. (2001) Improved PCR-Walking for large-scale isolation of plant T-DNA borders. BioTechniques, 30, 496-504.
Bechtold, N., Ellis J.and Pelletier G. (1993) In planta Agrobacterium mediated gene transfer by filtration of adult Arabidopsis thaliana plants. C. R. Acad. Sci. (Paris) Ser. III Sci. Vie, 316, 1194-1199.
Bouchez, D., Camilleri, C., and Caboche, M. (1993) A binary vector based on Basta resistance in planta transformation of Arabidopsis thaliana. C. R. Acad. Sci. (Paris) Ser. III Sci. Vie, 316, 88-1193.